Glycyrrhiza glabra (root)
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'''Mobile Phase:''' 0.14 g of anhydrous potassium dihydrogen phosphate in 900 mL of water, add 0.5 mL phosphoric acid, mix, complete to volume with water, and mix (Solution A); and acetonitrile (Solution B) | '''Mobile Phase:''' 0.14 g of anhydrous potassium dihydrogen phosphate in 900 mL of water, add 0.5 mL phosphoric acid, mix, complete to volume with water, and mix (Solution A); and acetonitrile (Solution B) | ||
− | '''Elution:''' gradient program, see below | + | '''Elution:''' gradient program, see Table below |
'''Flow rate:''' 1.5 mL/min | '''Flow rate:''' 1.5 mL/min | ||
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'''Detection:''' UV, 254 nm | '''Detection:''' UV, 254 nm | ||
− | '''Injection volume:''' 20 uL}} | + | '''Injection volume:''' 20 uL |
+ | |||
+ | {| | ||
+ | | Time (min) || Solution A (%) || Solution B (%) | ||
+ | |0-18 || 95-55 || 5-45 | ||
+ | |18-25 || 55-20 || 45-80 | ||
+ | |25-28 || 20 || 80 | ||
+ | |28-35 || 20-55 || 80-45 | ||
+ | |35-40 || 55-95 || 45-5 | ||
+ | |40-45 || 95 || 5 | ||
+ | |} | ||
+ | |||
+ | }} | ||
=High Performance Thin Layer Chromatographic Identification= | =High Performance Thin Layer Chromatographic Identification= |
Revision as of 15:23, 28 February 2014
Contents |
Nomenclature
Botanical Voucher Specimen
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Organoleptic Characteristics
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Macroscopic Characteristics
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Microscopic Characteristics
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High Performance Liquid Chromatographic Identification
'Sample Preparations: Extract 1.0 g of coarsely powdered Glycyrrhiza root in 50 mL of water by boiling for about 5 minutes, and filter. Repeat for 4-5 times or until the extract is colorless. Combine the extracts, concentrate to about 100 mL, and cool to room temperature. Before injection, filter through a membrane filter of 0.45-um or finer pore size, discarding the first 5 mL of the filtrate.
Column: C18, 25-cm x 4.6 mm, 5-um, XXXXX, XXXXX
Mobile Phase: 0.14 g of anhydrous potassium dihydrogen phosphate in 900 mL of water, add 0.5 mL phosphoric acid, mix, complete to volume with water, and mix (Solution A); and acetonitrile (Solution B)
Elution: gradient program, see Table below
Flow rate: 1.5 mL/min
Detection: UV, 254 nm
Injection volume: 20 uL
{
Source: Natural Remedies Pvt Ltd [14]
High Performance Thin Layer Chromatographic Identification
Supplementary Information
Sources
- ↑ Tropicos.org. Missouri Botanical Garden. 27 Feb 2014 <http://www.tropicos.org/Image/100253567>
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/
- ↑ American Herbal Products Association. March 2013. Organoleptic Analysis of Herbal Ingredients. AHPA: Silver Spring, MD http://www.ahpa.org/
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/
- ↑ Flora von Deutschland, Österreich und der Schweiz- Otto Wilhelm Thomé (1885)
- ↑ Encyclopedia of Life http://eol.org/data_objects/2447928
- ↑ Encyclopedia of Life http://eol.org/data_objects/24932881
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/
- ↑ Encyclopedia of Life http://eol.org/data_objects/19163752
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/
- ↑ Natural Remedies Pvt Ltd http://www.naturalremedy.com/