Handroanthus impetiginosus (bark)

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=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Pau D’Arco (bark) (''Handroanthus impetiginosus'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Handroanthus_impetiginosus_bark_-_Alkemists_Laboratories.jpg
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            | caption1=''Handroanthus impetiginosus'' HPTLC ID - 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=toluene: ethyl acetate: formic acid [7.5/2.5/0.5]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.
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            | detection=10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL ''Handroanthus impetiginosus''-1 (bark)
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# 3 μL ''Handroanthus impetiginosus''-2 (bark)
 +
# 3 μL ''Handroanthus impetiginosus''-3 (bark)
 +
# 3 μL ''Handroanthus impetiginosus''-4 (bark)
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# 3 μL ''Handroanthus impetiginosus''-4 (bark)
 +
# 3 μL ''Handroanthus impetiginosus''-5 (bark)
 +
# 3 μL ''Handroanthus impetiginosus''-6 (bark)
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# 1 μL Artemisinin ~0.1%  in CH3OH
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
 +
            | reference=Plant Drug Analysis, Wagner, H., 1996
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            | }}
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 +
 
=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:NoIntro]]
 
[[Category:NoIntro]]

Revision as of 22:13, 15 March 2013

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Fragment of the bark showing dispersal of medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen is the fragment of the bark showing dispersal of medullary rays and the bark fiber showing calcium oxalate prisms spaced by medullary rays when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Pau D'Arco Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Handroanthus impetiginosus HPTLC ID - 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm

Pau D’Arco (bark) (Handroanthus impetiginosus)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Handroanthus impetiginosus-1 (bark)
  2. 3 μL Handroanthus impetiginosus-2 (bark)
  3. 3 μL Handroanthus impetiginosus-3 (bark)
  4. 3 μL Handroanthus impetiginosus-4 (bark)
  5. 3 μL Handroanthus impetiginosus-4 (bark)
  6. 3 μL Handroanthus impetiginosus-5 (bark)
  7. 3 μL Handroanthus impetiginosus-6 (bark)
  8. 1 μL Artemisinin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate: formic acid [7.5/2.5/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Other Points of Interest


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