Handroanthus impetiginosus (bark)

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=Introduction=
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{{DISPLAYTITLE:''Handroanthus impetiginosus'' (bark) }} {{askbox|herb=''Handroanthus impetiginosus''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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=HPTLC Entries=
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=Botanical Voucher Specimen=
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=Other Points of Interest=
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{{media2 |cat=voucher
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[[Category:blank]]
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| source=Royal Botanic Gardens, Kew.
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| companyimage=Kewlogo.gif
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| companyURL=http://specimens.kew.org/herbarium/K000449912
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| mainimage=Handroanthus_impetiginosus_Kew_imageBarcode=K000449912_257528.jpg
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| source2=Royal Botanic Gardens, Kew.
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| companyimage2=Kewlogo.gif
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| companyURL2=http://specimens.kew.org/herbarium/K000449913
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| image2=Handroanthus_impetiginosus_Kew_imageBarcode=K000449913_257529.jpg
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}}
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=Organoleptic Characteristics=
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=Macroscopic Characteristics=
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=Microscopic Characteristics=
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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      | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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      | companyURL=http://www.alkemist.com
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      | mainimage=Pau D'Arco Alkemist Laboratories.jpg
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      | caption1=Fragment of the bark showing dispersal of medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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| source2=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2=http://www.alkemist.com
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      | image2=Pau D'Arco-1 Alkemist Laboratories.jpg
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      | caption2=Bark fiber showing calcium oxalate prisms spaced by medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | characteristics=cellular structures identified in this botanical specimen is the fragment of the bark showing dispersal of medullary rays and the bark fiber showing calcium oxalate prisms spaced by medullary rays when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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            | reference=Plant Drug Analysis, Wagner, H., 1996
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      | }}
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=High Performance Thin Layer Chromatographic Identification=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Pau D’Arco (bark) (''Handroanthus impetiginosus'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Handroanthus_impetiginosus_bark_-_Alkemists_Laboratories.jpg
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            | caption1=''Handroanthus impetiginosus'' HPTLC ID - 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=toluene: ethyl acetate: formic acid [7.5/2.5/0.5]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.
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            | detection=10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL ''Handroanthus impetiginosus''-1 (bark)
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# 3 μL ''Handroanthus impetiginosus''-2 (bark)
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# 3 μL ''Handroanthus impetiginosus''-3 (bark)
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# 3 μL ''Handroanthus impetiginosus''-4 (bark)
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# 3 μL ''Handroanthus impetiginosus''-4 (bark)
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# 3 μL ''Handroanthus impetiginosus''-5 (bark)
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# 3 μL ''Handroanthus impetiginosus''-6 (bark)
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# 1 μL Artemisinin ~0.1%  in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Plant Drug Analysis, Wagner, H., 1996
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            | }}
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=Supplementary Information=
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=Sources=
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<references />

Latest revision as of 02:27, 19 August 2014

AHPA recognizes other valuable resources exist regarding the identity of Handroanthus impetiginosus.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Botanical Voucher Specimen

bottomright bottomright

Handroanthus impetiginosus Kew imageBarcode=K000449912 257528.jpg
Source: Royal Botanic Gardens, Kew.[1]

Handroanthus impetiginosus Kew imageBarcode=K000449913 257529.jpg
Source: Royal Botanic Gardens, Kew.[2]


Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

bottomright bottomright

Pau D'Arco Alkemist Laboratories.jpg
Fragment of the bark showing dispersal of medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]

Pau D'Arco-1 Alkemist Laboratories.jpg
Bark fiber showing calcium oxalate prisms spaced by medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[4]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Handroanthus impetiginosus HPTLC ID - 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm

Pau D’Arco (bark) (Handroanthus impetiginosus)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Handroanthus impetiginosus-1 (bark)
  2. 3 μL Handroanthus impetiginosus-2 (bark)
  3. 3 μL Handroanthus impetiginosus-3 (bark)
  4. 3 μL Handroanthus impetiginosus-4 (bark)
  5. 3 μL Handroanthus impetiginosus-4 (bark)
  6. 3 μL Handroanthus impetiginosus-5 (bark)
  7. 3 μL Handroanthus impetiginosus-6 (bark)
  8. 1 μL Artemisinin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate: formic acid [7.5/2.5/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [5]


Supplementary Information

Sources

  1. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000449912
  2. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000449913
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  5. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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