Handroanthus impetiginosus (bark)

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AHPA recognizes other valuable resources exist regarding the identity of Handroanthus impetiginosus.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Botanical Voucher Specimen

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Handroanthus impetiginosus Kew imageBarcode=K000449912 257528.jpg
Source: Royal Botanic Gardens, Kew.[1]

Handroanthus impetiginosus Kew imageBarcode=K000449913 257529.jpg
Source: Royal Botanic Gardens, Kew.[2]


Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

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Pau D'Arco Alkemist Laboratories.jpg
Fragment of the bark showing dispersal of medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]

Pau D'Arco-1 Alkemist Laboratories.jpg
Bark fiber showing calcium oxalate prisms spaced by medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[4]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Handroanthus impetiginosus HPTLC ID - 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm

Pau D’Arco (bark) (Handroanthus impetiginosus)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Handroanthus impetiginosus-1 (bark)
  2. 3 μL Handroanthus impetiginosus-2 (bark)
  3. 3 μL Handroanthus impetiginosus-3 (bark)
  4. 3 μL Handroanthus impetiginosus-4 (bark)
  5. 3 μL Handroanthus impetiginosus-4 (bark)
  6. 3 μL Handroanthus impetiginosus-5 (bark)
  7. 3 μL Handroanthus impetiginosus-6 (bark)
  8. 1 μL Artemisinin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate: formic acid [7.5/2.5/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [5]


Supplementary Information

Sources

  1. Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000449912
  2. Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000449913
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  5. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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