Hibiscus sabdariffa (flower)

Microscopic Entries

Rosettes of Calcium oxalate with pink pigment observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the rosettes of calcium oxalate with pink pigment and the fragment of a thin walled trichome when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

HPTLC Entries

Hibiscus sabdariffa HPTLC ID - Thymol Sulfuric Acid Reagent UV 365 nm Hibiscus (flower) (Hibiscus sabdariffa) Lane Assignments Lanes, from left to right (Track, Volume, Sample): μL Epicatechin ~0.1% in CH3OH μL Hibiscus sabdariffa-1 (flower) μL Hibiscus sabdariffa-2 (flower) μL Hibiscus sabdariffa-3 (flower) μL Hibiscus sabdariffa-3 (flower) μL Hibiscus sabdariffa-4 (flower) μL Hibiscus sabdariffa-5 (flower) μL Catechin ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase tolulene: ethyl formate: formic acid [5/4/1]  Sample Preparation Method 0.5 g + 5mL 70%gr EtOH sonicate/heat @ 50° C~1/2 hr, evap w N2, qs 1mL CH3OH  Detection Method Thymol Sulfuric acid spray -> 115° C 10 min -> UV 365 nm  Reference see Thin-Layer Chromatography Jork, Funk Wimmer P421-424 Source: Elan M. Sudberg, Alkemist Laboratories