Hordeum vulgare (leaf)

From AHPA Botanical Identity References Compendium
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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Barley (leaf) (''Hordeum vulgare'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Hordeum_vulgare_-_Alkemists_Laboratories.jpg
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            | caption1=''Hordeum vulgare'' HPTLC ID - Natural Products + PEG UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr
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            | detection=Natural Product Reagent + PEG -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 1 μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH
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# 3 μL ''Hordeum vulgare''-1
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# 3 μL ''Hordeum vulgare''-2
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# 3 μL ''Hordeum vulgare''-3
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# 3 μL ''Hordeum vulgare''-3
 +
# 3 μL ''Hordeum vulgare''-4
 +
# 3 μL ''Hordeum vulgare''-5
 +
# 1 μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH
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 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.
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            | reference=Adapted from Plant Drug Analysis, Wagner, H., 1996
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 22:14, 22 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Small barb like trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are small barb like trichome and the epidermis showing long thin stomata when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Hordeum Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Hordeum vulgare HPTLC ID - Natural Products + PEG UV 365 nm

Barley (leaf) (Hordeum vulgare)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH
  2. 3 μL Hordeum vulgare-1
  3. 3 μL Hordeum vulgare-2
  4. 3 μL Hordeum vulgare-3
  5. 3 μL Hordeum vulgare-3
  6. 3 μL Hordeum vulgare-4
  7. 3 μL Hordeum vulgare-5
  8. 1 μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Adapted from Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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