Hordeum vulgare (leaf)
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| reference=Adapted from Plant Drug Analysis, Wagner, H., 1996 | | reference=Adapted from Plant Drug Analysis, Wagner, H., 1996 | ||
+ | | }} | ||
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+ | {{HPTLC | source=HPTLC Association | ||
+ | | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png | ||
+ | | companyURL=http://www.hptlc-association.org/ | ||
+ | | mainimage=Hordeum vulgare leaf-hptlc-association.png | ||
+ | | caption1=''Hordeum vulgare'' (leaf) HPTLC ID - NP/PEG reagent, UV 366 nm | ||
+ | | description=Barley grass (leaf) (''Hordeum vulgare'') | ||
+ | | | ||
+ | | stationaryphase=Stationary phase, i.e. Silica gel 60, F254 | ||
+ | | mobilephase= Formic acid, water, methyl ethyl ketone, ethyl acetate 10:10:30:50 (v/v/v/v) | ||
+ | | prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. | ||
+ | |||
+ | Derivatization reagent: 1.) NP reagent | ||
+ | Preparation: 1 g of natural products reagent in 200 mL ethyl acetate | ||
+ | 2.) PEG reagent | ||
+ | Preparation: 10 g of polyethylene glycol 400 in 200 mL dichloromethane | ||
+ | Use: Heat plate 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent | ||
+ | |||
+ | | detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% | ||
+ | | referencesamples=Reference: Dissolve 1 mg of rutin in 1 mL of methanol. Dissolve 1 mg of hyperoside in 1 mL of methanol. | ||
+ | | | ||
+ | | lanes=Lanes, from left to right (Track, Volume, Sample): | ||
+ | # 4 µL Rutin | ||
+ | # 4 µL Hyperoside | ||
+ | # 7 µL Oat herb 1 | ||
+ | # 10 µL Oat herb 1 | ||
+ | # 15 µL Oat herb 1 | ||
+ | # 10 µL Oat herb 2 | ||
+ | # 7 µL Barley grass 1 | ||
+ | # '''10 µL Barley grass 1''' | ||
+ | # 15 µL Barley grass 1 | ||
+ | # '''10 µL Barley grass 2''' | ||
+ | # 7 µL Wheat grass 1 | ||
+ | # 10 µL Wheat grass 1 | ||
+ | # 15 µL Wheat grass 1 | ||
+ | # 10 µL Wheat grass 2 | ||
+ | |||
+ | | notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.'' | ||
+ | System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.32; Hyperoside: orange fluorescent zone at Rf ~ 0.50 | ||
+ | |||
+ | Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a yellow zone at Rf ~ 0.18 (red arrow). Above it there are several faint orange zones. There may be an orange zone at the position of reference rutin. A prominent red zone is located close to the solvent front. | ||
+ | |||
+ | Test for adulteration: No blue or green zone is seen between the position of references rutin and hyperoside (Oat herb, yellow arrow). No green zone is seen at Rf ~ 0.44 (Wheat grass, blue arrow). | ||
| }} | | }} | ||
=Other Points of Interest= | =Other Points of Interest= | ||
[[Category:NoIntro]] | [[Category:NoIntro]] |
Revision as of 18:11, 21 June 2013
Contents |
Introduction
Macroscopic Entries
Microscopic Entries
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HPTLC Entries
Barley (leaf) (Hordeum vulgare) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates Mobile Phase ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4] Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr Detection Method Natural Product Reagent + PEG -> UV 365 nm Reference see Adapted from Plant Drug Analysis, Wagner, H., 1996
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Barley grass (leaf) (Hordeum vulgare) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference Sample(s) Reference: Dissolve 1 mg of rutin in 1 mL of methanol. Dissolve 1 mg of hyperoside in 1 mL of methanol. Stationary Phase Stationary phase, i.e. Silica gel 60, F254 Mobile Phase Formic acid, water, methyl ethyl ketone, ethyl acetate 10:10:30:50 (v/v/v/v) Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Derivatization reagent: 1.) NP reagent Preparation: 1 g of natural products reagent in 200 mL ethyl acetate 2.) PEG reagent Preparation: 10 g of polyethylene glycol 400 in 200 mL dichloromethane Use: Heat plate 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes. System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.32; Hyperoside: orange fluorescent zone at Rf ~ 0.50 Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a yellow zone at Rf ~ 0.18 (red arrow). Above it there are several faint orange zones. There may be an orange zone at the position of reference rutin. A prominent red zone is located close to the solvent front. Test for adulteration: No blue or green zone is seen between the position of references rutin and hyperoside (Oat herb, yellow arrow). No green zone is seen at Rf ~ 0.44 (Wheat grass, blue arrow).
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Other Points of Interest
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