Revision as of 04:57, 27 February 2014

Nomenclature

Hydrastis canadensis L.   Ranunculaceae  
Standardized common name (English): goldenseal

Introduction

Introduction from Wikipedia, the free encyclopedia (http://en.wikipedia.org/wiki/Hydrastis_canadensis, retrieved 02/27/2012).

Goldenseal (Orange-root, Orangeroot; Hydrastis canadensis) is a perennial herb in the buttercup family Ranunculaceae, native to southeastern Canada and the northeastern United States. It may be distinguished by its thick, yellow knotted rootstock. The stem is purplish and hairy above ground and yellow below ground where it connects to the yellow rhizome. The plant bears two palmate, hairy leaves with 5–7 double-toothed lobes and single, small, inconspicuous flowers with greenish white stamens in the late spring. It bears a single berry like a large raspberry with 10–30 seeds in the summer.

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Macroscopic Entries

Microscopic Entries

Vessel element showing circular perforation from Hydrastis canadensis viewed at 400x with Acidified Chloral Hydrate.cellular structures identified in Hydrastis canadensis are the vessel element showing circular perforation and the orange brown granular mass when observed at 400x with Acidified Chloral Hydrate. Source: Elan M. Sudberg, Alkemist Laboratories [1]

HPTLC Entries

Hydrastis canadensis HPTLC ID - Ninhydrin Reagent UV 365 nm Golden Seal (root) (Hydrastis canadensis) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 5 μL Hydrastine ~0.1% in Methanol 3 μL Hydrastis canadensis-1 (root) 3 μL Hydrastis canadensis-2 (root) 3 μL Hydrastis canadensis-3 (root) 3 μL Hydrastis canadensis-3 (root) 3 μL Hydrastis canadensis-4 (root) 3 μL Hydrastis canadensis-5 (root) 1 μL Berberine chloride ~0.1% in Methanol Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase ethyl acetate: methanol: HCOOH: water [10/2/1.2/0.6]  Sample Preparation Method 0.25g+4 ml 80% MeOH snct 1/2 hr cntrfg/dcnt, add 2mL MeOH, vrtx&cntrfg,dcnt&qs to 20mL w MeOH  Detection Method Ninhydrin Reagent -> UV 365 nm  Reference see American Herbal Pharmacopoeia & Therapeutic Compendium Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest

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