Hydrastis canadensis (root)

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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
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Revision as of 23:56, 15 March 2014

Contents

Nomenclature

Hydrastis canadensis L.   Ranunculaceae  
Standardized common name (English): goldenseal

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

Vessel element showing circular perforation from Hydrastis canadensis viewed at 400x with Acidified Chloral Hydrate.cellular structures identified in Hydrastis canadensis are the vessel element showing circular perforation and the orange brown granular mass when observed at 400x with Acidified Chloral Hydrate.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Hydrastis canadensis L. -Ranunculaceae--1.jpg


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Hydrastis canadensis HPTLC ID - Ninhydrin Reagent UV 365 nm

Golden Seal (root) (Hydrastis canadensis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 5 μL Hydrastine ~0.1% in Methanol
  2. 3 μL Hydrastis canadensis-1 (root)
  3. 3 μL Hydrastis canadensis-2 (root)
  4. 3 μL Hydrastis canadensis-3 (root)
  5. 3 μL Hydrastis canadensis-3 (root)
  6. 3 μL Hydrastis canadensis-4 (root)
  7. 3 μL Hydrastis canadensis-5 (root)
  8. 1 μL Berberine chloride ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: methanol: HCOOH: water [10/2/1.2/0.6] 

Sample Preparation Method 0.25g+4 ml 80% MeOH snct 1/2 hr cntrfg/dcnt, add 2mL MeOH, vrtx&cntrfg,dcnt&qs to 20mL w MeOH 

Detection Method Ninhydrin Reagent -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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