Source: MOBOT, Tropicos.org^[1]

Source: PlantaPhile^[2]

Source: PlantaPhile^[3]

Vessel element showing circular perforation from Hydrastis canadensis viewed at 400x with Acidified Chloral Hydrate.
Source: Elan M. Sudberg, Alkemist Laboratories^[4]

Orange brown granular mass from Hydrastis canadensis viewed at 400x with Acidified Chloral Hydrate.
Source: Elan M. Sudberg, Alkemist Laboratories^[5]

Hydrastis canadensis HPTLC ID - Ninhydrin Reagent UV 365 nm

Golden Seal (root) (Hydrastis canadensis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 5 μL Hydrastine ~0.1% in Methanol
2. 3 μL Hydrastis canadensis-1 (root)
3. 3 μL Hydrastis canadensis-2 (root)
4. 3 μL Hydrastis canadensis-3 (root)
5. 3 μL Hydrastis canadensis-3 (root)
6. 3 μL Hydrastis canadensis-4 (root)
7. 3 μL Hydrastis canadensis-5 (root)
8. 1 μL Berberine chloride ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: methanol: HCOOH: water [10/2/1.2/0.6] 

Sample Preparation Method 0.25g+4 ml 80% MeOH snct 1/2 hr cntrfg/dcnt, add 2mL MeOH, vrtx&cntrfg,dcnt&qs to 20mL w MeOH 

Detection Method Ninhydrin Reagent -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium

Source: Elan M. Sudberg, Alkemist Laboratories ^[6]