Hydrastis canadensis (root)

Nomenclature

Hydrastis canadensis L.   Ranunculaceae  
Standardized common name (English): goldenseal

Microscopic Characteristics

Vessel element showing circular perforation from Hydrastis canadensis viewed at 400x with Acidified Chloral Hydrate.cellular structures identified in Hydrastis canadensis are the vessel element showing circular perforation and the orange brown granular mass when observed at 400x with Acidified Chloral Hydrate. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Hydrastis canadensis HPTLC ID - Ninhydrin Reagent UV 365 nm Golden Seal (root) (Hydrastis canadensis) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 5 μL Hydrastine ~0.1% in Methanol 3 μL Hydrastis canadensis-1 (root) 3 μL Hydrastis canadensis-2 (root) 3 μL Hydrastis canadensis-3 (root) 3 μL Hydrastis canadensis-3 (root) 3 μL Hydrastis canadensis-4 (root) 3 μL Hydrastis canadensis-5 (root) 1 μL Berberine chloride ~0.1% in Methanol Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase ethyl acetate: methanol: HCOOH: water [10/2/1.2/0.6]  Sample Preparation Method 0.25g+4 ml 80% MeOH snct 1/2 hr cntrfg/dcnt, add 2mL MeOH, vrtx&cntrfg,dcnt&qs to 20mL w MeOH  Detection Method Ninhydrin Reagent -> UV 365 nm  Reference see American Herbal Pharmacopoeia & Therapeutic Compendium Source: Elan M. Sudberg, Alkemist Laboratories