Juglans nigra HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Black Walnut (Hull) (Juglans nigra)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 5 μL Catechin~0.1% in CH3OH
2. 5 μL Juglans nigra-1 (hull)
3. 5 μL Juglans nigra-2 (hull)
4. 5 μL Juglans nigra-3 (hull)
5. 5 μL Juglans nigra-4 (hull)
6. 5 μL Juglans nigra-4 (hull)
7. 5 μL Juglans nigra-5 (hull)
8. 5 μL Juglans nigra-6 (hull)

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethyl formate: formic acid [5/4/1] 

Sample Preparation Method 0.3g+3mL 50% grain EtOH sonicate/heat @~50° C ~ 1/2 hr. 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories

Source: Elan M. Sudberg, Alkemist Laboratories ^[1]