Juglans nigra (hull)

From AHPA Botanical Identity References Compendium
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=Introduction=
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{{DISPLAYTITLE:''Juglans nigra'' (hull) }} {{askbox|herb=''Juglans nigra''}}
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=Nomenclature=
  
=Macroscopic Entries=
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{{nomenclature | binomial=Juglans nigra
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|authority=L.
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|family=Juglandaceae
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|scn=black walnut
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|syn=
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|ayurvedic=
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|pinyin=
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|aka=
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|notes= }}
  
=Microscopic Entries=
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=Botanical Voucher Specimen=
  
=HPTLC Entries=
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{{Media      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Juglans_nigra_Tropicos_100276438.jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100276438
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            | reference=Tropicos.org. Missouri Botanical Garden. 25 Mar 2014 <http://www.tropicos.org/Image/100276438>
  
=Other Points of Interest=
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            | }}
  
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[[Category:Blank2]]
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=Organoleptic Characteristics=
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=Macroscopic Characteristics=
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=Microscopic Characteristics=
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=High Performance Thin Layer Chromatographic Identification=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Black Walnut (Hull) (''Juglans nigra'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Juglans_nigra_hull_-_Alkemists_Laboratories.jpg‎
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            | caption1=''Juglans nigra'' HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: ethyl formate: formic acid [5/4/1]
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            | prep=0.3g+3mL 50% grain EtOH sonicate/heat @~50° C ~ 1/2 hr.
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 5 μL Catechin~0.1% in CH3OH
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# 5 μL ''Juglans nigra''-1 (hull)
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# 5 μL ''Juglans nigra''-2 (hull)
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# 5 μL ''Juglans nigra''-3 (hull)
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# 5 μL ''Juglans nigra''-4 (hull)
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# 5 μL ''Juglans nigra''-4 (hull)
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# 5 μL ''Juglans nigra''-5 (hull)
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# 5 μL ''Juglans nigra''-6 (hull)
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Method Developed by Alkemists Laboratories
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            | }}
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=Supplementary Information=
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=Sources=
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<references />

Latest revision as of 20:31, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Juglans nigra.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Juglans nigra L.   Juglandaceae  
Standardized common name (English): black walnut

Botanical Voucher Specimen

bottomright

Juglans nigra Tropicos 100276438.jpg
Source: MOBOT, Tropicos.org[1]


Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Juglans nigra HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Black Walnut (Hull) (Juglans nigra)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 5 μL Catechin~0.1% in CH3OH
  2. 5 μL Juglans nigra-1 (hull)
  3. 5 μL Juglans nigra-2 (hull)
  4. 5 μL Juglans nigra-3 (hull)
  5. 5 μL Juglans nigra-4 (hull)
  6. 5 μL Juglans nigra-4 (hull)
  7. 5 μL Juglans nigra-5 (hull)
  8. 5 μL Juglans nigra-6 (hull)

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethyl formate: formic acid [5/4/1] 

Sample Preparation Method 0.3g+3mL 50% grain EtOH sonicate/heat @~50° C ~ 1/2 hr. 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100276438
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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