Lactuca virosa (leaf)

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=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Wild Lettuce (leaf) (''Lactuca virosa'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Lactuca_virosa_-_Alkemists_Laboratories.jpg
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            | caption1=''Lactuca virosa'' HPTLC ID - Natural Product Reagent + PEG UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=ethyl acetate: formic acid: glacial acetic acid: water [10/0.9/0.9/2]
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            | prep=0.3g+3mL CH3OH sonicate/heat @~50° C ~ 1/2 hr
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            | detection= Natural Product Reagent + PEG -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
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# 3 μL ''Lactuca virosa''-1 (leaf)
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# 3 μL ''Lactuca virosa''-2 (herb)
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# 3 μL ''Lactuca virosa''-3 (leaf)
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# 3 μL ''Lactuca virosa''-3 (leaf)
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# 3 μL ''Lactuca virosa''-4 (herb)
 +
# 3 μL ''Lactuca virosa''-5 (herb)
 +
# 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
 +
            | reference=Lactuca_virosa_-_Alkemists_Laboratories.jpg
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 23:00, 4 December 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Large trichome found on the lower epidermis near the vein showing lignified cell observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Lactuca virosa - Alkemist Laboratories.png


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Lactuca virosa HPTLC ID - Natural Product Reagent + PEG UV 365 nm

Wild Lettuce (leaf) (Lactuca virosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
  2. 3 μL Lactuca virosa-1 (leaf)
  3. 3 μL Lactuca virosa-2 (herb)
  4. 3 μL Lactuca virosa-3 (leaf)
  5. 3 μL Lactuca virosa-3 (leaf)
  6. 3 μL Lactuca virosa-4 (herb)
  7. 3 μL Lactuca virosa-5 (herb)
  8. 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: formic acid: glacial acetic acid: water [10/0.9/0.9/2] 

Sample Preparation Method 0.3g+3mL CH3OH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Lactuca_virosa_-_Alkemists_Laboratories.jpg


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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