Lactuca virosa (leaf)

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Revision as of 06:57, 25 March 2014

Contents

Nomenclature

Lactuca virosa L.   Asteraceae  
Standardized common name (English): wild lettuce

Botanical Voucher Specimen

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Lactuca virosa Tropicos 100263601.jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

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Lactuca virosa - Alkemist Laboratories.png
Large trichome found on the lower epidermis near the vein showing lignified cell observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Lactuca virosa-1- Alkemist Laboratories.png
Dark brown latex observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Lactuca virosa HPTLC ID - Natural Product Reagent + PEG UV 365 nm

Wild Lettuce (leaf) (Lactuca virosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
  2. 3 μL Lactuca virosa-1 (leaf)
  3. 3 μL Lactuca virosa-2 (herb)
  4. 3 μL Lactuca virosa-3 (leaf)
  5. 3 μL Lactuca virosa-3 (leaf)
  6. 3 μL Lactuca virosa-4 (herb)
  7. 3 μL Lactuca virosa-5 (herb)
  8. 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: formic acid: glacial acetic acid: water [10/0.9/0.9/2] 

Sample Preparation Method 0.3g+3mL CH3OH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Lactuca_virosa_-_Alkemists_Laboratories.jpg


Source: Elan M. Sudberg, Alkemist Laboratories [4]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100263601
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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