Lactuca virosa (leaf)

Nomenclature

Lactuca virosa L.   Asteraceae  
Standardized common name (English): wild lettuce

Botanical Voucher Specimen

Source: MOBOT, Tropicos.org	Source: Royal Botanic Gardens, Kew.	Lactuca virosa var. cruenta Source: Royal Botanic Gardens, Kew.

Microscopic Characteristics

Large trichome found on the lower epidermis near the vein showing lignified cell observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories	Dark brown latex observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Lactuca virosa HPTLC ID - Natural Product Reagent + PEG UV 365 nm Wild Lettuce (leaf) (Lactuca virosa) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol 3 μL Lactuca virosa-1 (leaf) 3 μL Lactuca virosa-2 (herb) 3 μL Lactuca virosa-3 (leaf) 3 μL Lactuca virosa-3 (leaf) 3 μL Lactuca virosa-4 (herb) 3 μL Lactuca virosa-5 (herb) 2 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase ethyl acetate: formic acid: glacial acetic acid: water [10/0.9/0.9/2]  Sample Preparation Method 0.3g+3mL CH3OH sonicate/heat @~50° C ~ 1/2 hr  Detection Method Natural Product Reagent + PEG -> UV 365 nm  Reference see Lactuca_virosa_-_Alkemists_Laboratories.jpg Source: Elan M. Sudberg, Alkemist Laboratories