Lycium barbarum HPTLC ID - Vanillin Sulfuric Acid Reagent - UV 365 nm

Goji (fruit) (Lycium barbarum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 2 μL Catechin ~0.1% in Methanol
2. 3 μL Lycium chinense-1 (fruit)
3. 3 μL Lycium barbarum-1 (fruit)
4. 3 μL Lycium barbarum-2 (fruit)
5. 3 μL Lycium barbarum-2 (fruit)
6. 3 μL Lycium barbarum-3 (fruit)
7. 3 μL Lycium barbarum-4 (fruit)
8. 2 μL Gallic Acid ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: CHCl3: HCOOH [6/4/2] 

Sample Preparation Method 0.3 g + 3 ml 80% CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]