Magnolia officinalis HPTLC ID - 10% Ethanolic H2SO4 visible light

Magnolia (bark) (Magnolia officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Magnolol ~ 0.1% in Methanol
2. 3 μL Magnolia officinalis-1
3. 3 μL Magnolia officinalis-2
4. 3 μL Magnolia officinalis-3
5. 3 μL Magnolia officinalis-3
6. 4 μL Magnolia officinalis-4
7. 2 μL Magnolia officinalis-4
8. 3 μL Honokiol ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase cyclohexane: ethyl acetate: HCOOH [6/4/0.2] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method 10% Ethanolic H2SO4 -> 115° C 15 min -> visible light 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 1997

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]