Malus pumila (fruit)

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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Apple (fruit) (''Malus pumila'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Malus pumila fruit - Alkemists Laboratories.jpg
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            | caption1=''Malus pumila'' HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=CHCl3: ethyl formate: HCOOH [6/1.5/1.5]
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            | prep=0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection= Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL ''Malus pumila''-1 (entire)
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# 3 μL ''Malus pumila''-2 (fruit)
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# 3 μL ''Malus pumila''-3 (fruit)
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# 3 μL ''Malus pumila''-4 (fruit)
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# 3 μL ''Malus pumila''-4 (fruit)
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# 3 μL ''Malus pumila''-5 (fruit)
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# 3 μL ''Malus pumila''-6 (peel)
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# 3 μL Catechin ~0.1% in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Method Developed by Alkemists Laboratories
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            | }}
  
 
=Other Points of Interest=
 
=Other Points of Interest=

Revision as of 21:56, 15 March 2013

Contents

Introduction

Macroscopic Entries

Microscopic Entries

HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Malus pumila HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Apple (fruit) (Malus pumila)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Malus pumila-1 (entire)
  2. 3 μL Malus pumila-2 (fruit)
  3. 3 μL Malus pumila-3 (fruit)
  4. 3 μL Malus pumila-4 (fruit)
  5. 3 μL Malus pumila-4 (fruit)
  6. 3 μL Malus pumila-5 (fruit)
  7. 3 μL Malus pumila-6 (peel)
  8. 3 μL Catechin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: ethyl formate: HCOOH [6/1.5/1.5] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [1]

Other Points of Interest


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