Malva sylvestris (flower)
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=HPTLC Entries= | =HPTLC Entries= | ||
+ | {{HPTLC | source=HPTLC Association | ||
+ | | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png | ||
+ | | companyURL=http://www.hptlc-association.org/ | ||
+ | | mainimage=Malva sylvestris-hptclc-association.png | ||
+ | | caption1=Mallow (flower) HPTLC ID - White RT | ||
+ | | description=Mallow (flower) (''Malva sylvestris'') | ||
+ | | | ||
+ | | stationaryphase=Stationary phase, i.e. Silica gel 60, F254 | ||
+ | | mobilephase=1-Butanol, formic acid, water 65:16:19 (v/v/v) | ||
+ | | prep=Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. | ||
+ | |||
+ | | detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% | ||
+ | | referencesamples=Reference: Dissolve 2 mg of pelargonin in 5 mL of methanol. Dissolve 2 mg of delphinidin in 5 mL of methanol; Optional: dissolve 2 mg of malvidin in 5 mL of methanol. | ||
+ | | | ||
+ | | lanes=Lanes, from left to right (Track, Volume, Sample): | ||
+ | # 2 µL Bilberry fruit (Ph.Eur. extraction) | ||
+ | # 3 µL Bilberry fruit (Ph.Eur. extraction) | ||
+ | # 4 µL Bilberry fruit (Ph.Eur. extraction) | ||
+ | # 2 µL Bilberry fruit | ||
+ | # 3 µL Bilberry fruit | ||
+ | # 4 µL Bilberry fruit | ||
+ | # 2 µL Pelargonin | ||
+ | # 2 µL Malvidin | ||
+ | # 2 µL Delphinidin | ||
+ | # 2 µL Mallow flower | ||
+ | # '''4 µL Mallow flower''' | ||
+ | # 6 µL Mallow flower | ||
+ | # 2 µL Roselle flower | ||
+ | # 4 µL Roselle flower | ||
+ | # 6 µL Roselle flower | ||
+ | |||
+ | | notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.'' | ||
+ | |||
+ | System suitability test: Pelargonin: red to orange zone at Rf ~ 0.89; Delphinidin: violet zone at Rf ~ 0.80 | ||
+ | |||
+ | Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows one intensive violet zone at Rf ~ 0.35. | ||
+ | |||
+ | Test for other species: No intense violet-blue zones are seen between Rf ~ 0.46 and 0.55 (Bilberry fruit) and no violet zone is present at Rf ~ 0.44 (Roselle flower). | ||
+ | | }} | ||
=Other Points of Interest= | =Other Points of Interest= | ||
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories | {{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories |
Revision as of 18:40, 21 June 2013
Contents |
Introduction
Macroscopic Entries
Microscopic Entries
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HPTLC Entries
Mallow (flower) (Malva sylvestris) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference Sample(s) Reference: Dissolve 2 mg of pelargonin in 5 mL of methanol. Dissolve 2 mg of delphinidin in 5 mL of methanol; Optional: dissolve 2 mg of malvidin in 5 mL of methanol. Stationary Phase Stationary phase, i.e. Silica gel 60, F254 Mobile Phase 1-Butanol, formic acid, water 65:16:19 (v/v/v) Sample Preparation Method Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes. System suitability test: Pelargonin: red to orange zone at Rf ~ 0.89; Delphinidin: violet zone at Rf ~ 0.80 Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows one intensive violet zone at Rf ~ 0.35. Test for other species: No intense violet-blue zones are seen between Rf ~ 0.46 and 0.55 (Bilberry fruit) and no violet zone is present at Rf ~ 0.44 (Roselle flower).
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Other Points of Interest
High Mallow (flower) (Malva sylvestris) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates Mobile Phase ethyl acetate: HCOOH: AcCOOH: H2O [10/1.1/1.1/2.4] Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr Detection Method Natural Product Reagent + PEG -> UV 365 nm Reference see Adapted from British Pharmacopoeia, 2003
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