Malva sylvestris HPTLC ID - Natural Product Reagent + PEG UV 365 nm

High Mallow (flower) (Malva sylvestris)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH
2. μL Malva sylvestris-1 (flower)
3. μL Malva sylvestris-1 (flower)
4. μL Malva sylvestris-2 (flower)
5. μL Malva sylvestris-2 (flower)
6. μL Malva sylvestris-3 (flower)
7. μL Malva sylvestris-4 Vouchered Specimen (flower)
8. μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: HCOOH: AcCOOH: H2O [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Adapted from British Pharmacopoeia, 2003

Source: Elan M. Sudberg, Alkemist Laboratories ^[4]

Mallow (flower) HPTLC ID - White RT

Mallow (flower) (Malva sylvestris)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 2 µL Bilberry fruit (Ph.Eur. extraction)
2. 3 µL Bilberry fruit (Ph.Eur. extraction)
3. 4 µL Bilberry fruit (Ph.Eur. extraction)
4. 2 µL Bilberry fruit
5. 3 µL Bilberry fruit
6. 4 µL Bilberry fruit
7. 2 µL Pelargonin
8. 2 µL Malvidin
9. 2 µL Delphinidin
10. 2 µL Mallow flower
11. 4 µL Mallow flower
12. 6 µL Mallow flower
13. 2 µL Roselle flower
14. 4 µL Roselle flower
15. 6 µL Roselle flower 

Reference Sample(s) Reference: Dissolve 2 mg of pelargonin in 5 mL of methanol. Dissolve 2 mg of delphinidin in 5 mL of methanol; Optional: dissolve 2 mg of malvidin in 5 mL of methanol. 

Stationary Phase Stationary phase, i.e. Silica gel 60, F254 

Mobile Phase 1-Butanol, formic acid, water 65:16:19 (v/v/v) 

Sample Preparation Method Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. 

Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test: Pelargonin: red to orange zone at Rf ~ 0.89; Delphinidin: violet zone at Rf ~ 0.80

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows one intensive violet zone at Rf ~ 0.35.

Test for other species: No intense violet-blue zones are seen between Rf ~ 0.46 and 0.55 (Bilberry fruit) and no violet zone is present at Rf ~ 0.44 (Roselle flower).

Source: HPTLC Association ^[5]