Revision as of 06:46, 23 January 2013 by
JKim (Talk)
Introduction
Macroscopic Entries
Microscopic Entries
Spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine and the thick walled stellate trichomes when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories [1]
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HPTLC Entries
Other Points of Interest
Malva sylvestris HPTLC ID - Natural Product Reagent + PEG UV 365 nm
High Mallow (flower) (Malva sylvestris)
Lane Assignments Lanes, from left to right (Track, Volume, Sample):
- μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH
- μL Malva sylvestris-1 (flower)
- μL Malva sylvestris-1 (flower)
- μL Malva sylvestris-2 (flower)
- μL Malva sylvestris-2 (flower)
- μL Malva sylvestris-3 (flower)
- μL Malva sylvestris-4 Vouchered Specimen (flower)
- μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.
Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates
Mobile Phase ethyl acetate: HCOOH: AcCOOH: H2O [10/1.1/1.1/2.4]
Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr
Detection Method Natural Product Reagent + PEG -> UV 365 nm
Reference see Adapted from British Pharmacopoeia, 2003
Source: Elan M. Sudberg, Alkemist Laboratories [2]
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