Matricaria recutita (flower)
Line 3: | Line 3: | ||
=Botanical Voucher Specimen= | =Botanical Voucher Specimen= | ||
− | |||
=Organoleptic Characteristics= | =Organoleptic Characteristics= | ||
Line 29: | Line 28: | ||
=Microscopic Characteristics= | =Microscopic Characteristics= | ||
− | + | {| border=1 | |
+ | | | ||
{{Microscopy | source=Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed. | {{Microscopy | source=Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed. | ||
| description=Chamomile (flowering parts) (''Matricaria chamomilla'') L., Compositae. | | description=Chamomile (flowering parts) (''Matricaria chamomilla'') L., Compositae. | ||
| color=Greenish. | | color=Greenish. | ||
− | |||
− | |||
| characteristics=Epidermal cells with sinuate vertical walls; abundant collapsed parenchyma tissue; very porous tracheid-like cells. No starch. The pollen grains with prominent conical projections and three pores form the most marked microscopial characteristic of the powder. The glandular trichromes are usually so much collapsed as to be unrecognizable. | | characteristics=Epidermal cells with sinuate vertical walls; abundant collapsed parenchyma tissue; very porous tracheid-like cells. No starch. The pollen grains with prominent conical projections and three pores form the most marked microscopial characteristic of the powder. The glandular trichromes are usually so much collapsed as to be unrecognizable. | ||
| ash=Ash 10 per cent. | | ash=Ash 10 per cent. | ||
Line 40: | Line 38: | ||
| }} | | }} | ||
+ | {| border=1 | ||
+ | | {{botcon |companyimage= WWWWWWWWWWWWWWWW | ||
+ | | companyURL= WWWWWWWWWWWWWWWW }} || {{botcon |companyimage= WWWWWWWWWWWWWWWW | ||
+ | | companyURL= WWWWWWWWWWWWWWWW }} | ||
+ | |- | ||
+ | | {{botimg |mainimage= WWWWWWWWWWWWWWWW | ||
+ | |caption1= WWWWWWWWWWWWWWWW | ||
+ | |source= WWWWWWWWWWWWWWWW }} | ||
+ | | {{botimg |mainimage= WWWWWWWWWWWWWWWW | ||
+ | |caption1= WWWWWWWWWWWWWWWW | ||
+ | |source= WWWWWWWWWWWWWWWW }} | ||
+ | |} | ||
+ | |||
+ | {| border=1 | ||
+ | | {{botcon |companyimage= WWWWWWWWWWWWWWWW | ||
+ | | companyURL= WWWWWWWWWWWWWWWW }} || {{botcon |companyimage= WWWWWWWWWWWWWWWW | ||
+ | | companyURL= WWWWWWWWWWWWWWWW }} | ||
+ | |- | ||
+ | | {{botimg |mainimage= WWWWWWWWWWWWWWWW | ||
+ | |caption1= WWWWWWWWWWWWWWWW | ||
+ | |source= WWWWWWWWWWWWWWWW }} | ||
+ | | {{botimg |mainimage= WWWWWWWWWWWWWWWW | ||
+ | |caption1= WWWWWWWWWWWWWWWW | ||
+ | |source= WWWWWWWWWWWWWWWW }} | ||
+ | |} | ||
{{Microscopy | source=Amy Brush, Traditional Medicinals | {{Microscopy | source=Amy Brush, Traditional Medicinals | ||
| companyimage=TMLogoK832X75.jpg | | companyimage=TMLogoK832X75.jpg | ||
Line 75: | Line 98: | ||
| caption10=Chamomile outer epidermis and oil, glycerine : deionized water, 400x | | caption10=Chamomile outer epidermis and oil, glycerine : deionized water, 400x | ||
| characteristics=epidermis has irregularly sinuous walls, often with palisade parenchmya, stomata deeply sunken surrounded by 6-8 subsidiary cells, various sizes of calcium oxalate cluster crystals abundant| }} | | characteristics=epidermis has irregularly sinuous walls, often with palisade parenchmya, stomata deeply sunken surrounded by 6-8 subsidiary cells, various sizes of calcium oxalate cluster crystals abundant| }} | ||
+ | |} | ||
=High Performance Thin Layer Chromatographic Identification= | =High Performance Thin Layer Chromatographic Identification= |
Revision as of 16:34, 18 February 2014
Contents |
Nomenclature
Botanical Voucher Specimen
Organoleptic Characteristics
|
|
Macroscopic Descriptions
|
Microscopic Characteristics
|
High Performance Thin Layer Chromatographic Identification
Chamomile (flower) (Matricaria recutita) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference Sample(s) Reference: Dissolve 1.5 mg of apigenin in 5 mL of methanol. Dissolve 1 mg of parthenolide in 5 mL of methanol. Stationary Phase Stationary phase, i.e. Silica gel 60, F254 Mobile Phase Ethyl acetate, cyclohexane 1:1 (v/v) Sample Preparation Method Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Dissolve 10 µL of the essential oil in 1 mL of toluene. Derivatization reagent: Anisaldehyde reagent; Preparation: 170 mL of ice-cooled methanol are mixed with 20 mL of acetic acid, 10 mL of sulfuric acid and 1 mL of anisaldehyde; Use: Dip (time 0, speed 5), heat at 100°C for 4 min. Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes. System suitability test (UV 366 nm): Apigenin: blue zone at Rf ~ 0.20; Parthenolide: pink zone at Rf ~ 0.48 Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 366 nm the chromatogram of the test solution shows a dark blue zone at Rf ~ 0.46 (blue arrow; not visible under white light) a brown zone between Rf ~ 0.30 and 0.40 and another weak blue zone at Rf ~ 0.30. At the position of apigenin reference substance (Rf ~ 0.20) two brown zones are present (violet under white light). Test for adulteration: Under UV 366 nm no pink zone (violet zone under white RT) at Rf ~ 0.48 corresponding to reference substance parthenolide is seen and there are no brown zones at the position of apigenin at Rf ~ 0.20 (green arrows, Feverfew flower). Under UV 366 nm there are no intense red zones (violet zones under white RT) between Rf ~ 0.20 and 0.30. No blue zone is seen at the position of parthenolide (orange arrows; Feverfew flower from Mexico). Under UV 366 nm no blue zone at the position of apigenin (Rf ~ 0.20) and no orange zone at Rf ~ 0.70 (purple under white light) is seen (yellow arrows; Roman Chamomile flower). Chamomile flower oil does not show any zones below Rf ~ 0.60. Source: HPTLC Association [10] |
Supplementary Information
Sources
- ↑ Steven Yeager, Mountain Rose Herbs http://www.MountainRoseHerbs.com
- ↑ Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed.
- ↑ Steven Yeager, Mountain Rose Herbs http://www.MountainRoseHerbs.com
- ↑ Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed.
- ↑ WWWWWWWWWWWWWWWW
- ↑ WWWWWWWWWWWWWWWW
- ↑ WWWWWWWWWWWWWWWW
- ↑ WWWWWWWWWWWWWWWW
- ↑ Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
- ↑ HPTLC Association http://www.hptlc-association.org/