Matricaria recutita (flower)

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           |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
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           |caption1= Chamomile papillose stigma with pollen grain, glycerine : deionized water, 600x
           |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
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           |source= Amy Brush, Traditional Medicinals  
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          |caption2= Chamomile inner (lower with pollen) and outer (upper with oil droplets), glycerine : deionized water, 200x6
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          |source2= Amy Brush, Traditional Medicinals
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|image3= chamomile_microrosettes,_600x.jpg
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          |caption3= Calcium oxalate microrosette crystals in chamomile, glycerine : deionized water, polarized light, 600x
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          |source3= Amy Brush, Traditional Medicinals
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          |caption1= Chamomile inner (lower with pollen) and outer (upper with oil droplets), glycerine : deionized water, 200x6
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          |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
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| {{botimg |mainimage= chamomile_microrosettes,_600x.jpg
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          |caption1= Calcium oxalate microrosette crystals in chamomile, glycerine : deionized water, polarized light, 600x
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          |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
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          |caption1= Outer epidermis with oil droplets, glycerine : deionized water, 200x
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          |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
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           |caption1= Pollen tubes in chamomile flower, glycerine : deionized water, 40x
 
           |caption1= Pollen tubes in chamomile flower, glycerine : deionized water, 40x
           |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
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           |caption1= Chamomile outer epidermis and oil, glycerine : deionized water, 400x
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           |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
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Revision as of 18:22, 19 March 2014

Contents

Nomenclature

Matricaria recutita L.   Asteraceae  
Syn. Chamomilla recutita (L.) Rauschert; Matricaria chamomilla L.; Matricaria suaveolens L.  
Standardized common name (English): chamomile

Botanical Voucher Specimen

Organoleptic Characteristics

Flavor: Slightly bitter, sweet, aromatic.

Aroma: Fragrant, aromatic, pleasant.

Source: Steven Yeager, Mountain Rose Herbs [1]

Mrh logo.jpg
Flavor: Bitter.

Aroma: Fragrant, chamomile odor.

Source: Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed. [2]

Macroscopic Characteristics

Flowers yellow, white, and some green; receptacle conical, hollow when cut longitudinally; phyllaries with pale dry margins.

Source: Steven Yeager, Mountain Rose Herbs [3]

Mrh logo.jpg


Microscopic Characteristics

Chamomile (flowering parts) (Matricaria chamomilla) L., Compositae.Epidermal cells with sinuate vertical walls; abundant collapsed parenchyma tissue; very porous tracheid-like cells. No starch. The pollen grains with prominent conical projections and three pores form the most marked microscopial characteristic of the powder. The glandular trichromes are usually so much collapsed as to be unrecognizable.

Source: Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed. [4]


Epidermis has irregularly sinuous walls, often with palisade parenchmya, stomata deeply sunken surrounded by 6-8 subsidiary cells, various sizes of calcium oxalate cluster crystals abundant.

Source: Amy Brush, Traditional Medicinals [5]

TMLogoK832X75.jpg


bottomright bottomright bottomright bottomright

Chamomile stigma, style and pollen, 200x.jpg
Chamomile papillose stigma, style and pollen grain, glycerine : deionized water, 200x
Source: Amy Brush, Traditional Medicinals[6]

Chamomile stigma with papillae and oil, 400x.jpg
Chamomile papillose stigma with oil droplets, glycerine : deionized water, 400x
Source: Amy Brush, Traditional Medicinals[7]

Chamomile sclerous ring on ovary, 100x.jpg
Chamomile sclerous ring at ovary base, glycerine : deionized water,_100x
Source: Amy Brush, Traditional Medicinals[8]

Chamomile ovary base, 400x.jpg
Chamomile, close-up of sclerous ring at ovary base and tricolpate pollen grain, glycerine : deionized water, 400x
Source: Amy Brush, Traditional Medicinals[9]

bottomright bottomright bottomright bottomright

Chamomile stigma with papillae and pollen, 600x.jpg
Chamomile papillose stigma with pollen grain, glycerine : deionized water, 600x
Source: Amy Brush, Traditional Medicinals[10]

Chamomile inner & outer epidermis, 200x.jpg
Chamomile inner (lower with pollen) and outer (upper with oil droplets), glycerine : deionized water, 200x6
Source: Amy Brush, Traditional Medicinals[11]

Chamomile microrosettes, 600x.jpg
Calcium oxalate microrosette crystals in chamomile, glycerine : deionized water, polarized light, 600x
Source: Amy Brush, Traditional Medicinals[12]

Chamomile outer epidermis with oil, 400x.jpg
Outer epidermis with oil droplets, glycerine : deionized water, 200x
Source: Amy Brush, Traditional Medicinals[13]

bottomright bottomright

Chamomile flower, pollen tubes 40x.jpg
Pollen tubes in chamomile flower, glycerine : deionized water, 40x
Source: Amy Brush, Traditional Medicinals[14]

Chamomile outer epidermis and oil, 400x.jpg
Chamomile outer epidermis and oil, glycerine : deionized water, 400x
Source: Amy Brush, Traditional Medicinals[15]


High Performance Thin Layer Chromatographic Identification

HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
(thumbnail)
Matricaria recutita HPTLC ID - Anisaldehyde reagent, UV 366 nm

Chamomile (flower) (Matricaria recutita)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 µL Feverfew flower from Mexico
  2. 2 µL Feverfew flower from Mexico
  3. 4 µL Feverfew flower from Mexico
  4. 3 µL Feverfew flower
  5. 3.5 µL Feverfew flower
  6. 4 µL Feverfew flower
  7. 2 µL Parthenolide
  8. 2 µL Apigenin
  9. 1 µL Roman Chamomile flower
  10. 2 µL Roman Chamomile flower
  11. 4 µL Roman Chamomile flower
  12. 1 µL Chamomile flower
  13. 2 µL Chamomile flower
  14. 4 µL Chamomile flower
  15. 1 µL Chamomile flower oil 

Reference Sample(s) Reference: Dissolve 1.5 mg of apigenin in 5 mL of methanol. Dissolve 1 mg of parthenolide in 5 mL of methanol. 

Stationary Phase Stationary phase, i.e. Silica gel 60, F254 

Mobile Phase Ethyl acetate, cyclohexane 1:1 (v/v) 

Sample Preparation Method Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Dissolve 10 µL of the essential oil in 1 mL of toluene.

Derivatization reagent: Anisaldehyde reagent; Preparation: 170 mL of ice-cooled methanol are mixed with 20 mL of acetic acid, 10 mL of sulfuric acid and 1 mL of anisaldehyde; Use: Dip (time 0, speed 5), heat at 100°C for 4 min. 

Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test (UV 366 nm): Apigenin: blue zone at Rf ~ 0.20; Parthenolide: pink zone at Rf ~ 0.48

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 366 nm the chromatogram of the test solution shows a dark blue zone at Rf ~ 0.46 (blue arrow; not visible under white light) a brown zone between Rf ~ 0.30 and 0.40 and another weak blue zone at Rf ~ 0.30. At the position of apigenin reference substance (Rf ~ 0.20) two brown zones are present (violet under white light).

Test for adulteration: Under UV 366 nm no pink zone (violet zone under white RT) at Rf ~ 0.48 corresponding to reference substance parthenolide is seen and there are no brown zones at the position of apigenin at Rf ~ 0.20 (green arrows, Feverfew flower). Under UV 366 nm there are no intense red zones (violet zones under white RT) between Rf ~ 0.20 and 0.30. No blue zone is seen at the position of parthenolide (orange arrows; Feverfew flower from Mexico). Under UV 366 nm no blue zone at the position of apigenin (Rf ~ 0.20) and no orange zone at Rf ~ 0.70 (purple under white light) is seen (yellow arrows; Roman Chamomile flower). Chamomile flower oil does not show any zones below Rf ~ 0.60.

Source: HPTLC Association [16]

Supplementary Information

Sources

  1. Steven Yeager, Mountain Rose Herbs http://www.MountainRoseHerbs.com
  2. Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed.
  3. Steven Yeager, Mountain Rose Herbs http://www.MountainRoseHerbs.com
  4. Schneider, A. (1921) The Microanalysis of Powdered Vegetable Drugs, 2nd ed.
  5. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  6. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  7. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  8. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  9. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  10. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  11. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  12. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  13. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  14. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  15. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  16. HPTLC Association http://www.hptlc-association.org/
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