Mucuna pruriens (seed)

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=Supplementary Information=
 
=Supplementary Information=
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==Other Publications==
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===HPTLC L-Dopa Estimation, Modi, ''et al.'', 2008===
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'''Estimation of L-Dopa from Mucuna pruriens LINN and Formulations Containing M. pruriens by HPTLC Method,'''
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<blockquote>'''Abstract.'''
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A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the analysis of L-dopa in Mucuna pruriens seed extract and its formulations. The method involves densitometric evaluation of L-dopa after resolving it by HPTLC on silica gel plates with n-butanol–acetic acid–water (4.0+1.0+1.0, v/v) as the mobile phase. Densitometric analysis of L-dopa was carried out in the absorbance mode at 280 nm. The relationship between the concentration of L-dopa and corresponding peak areas was found to be linear in the range of 100 to 1200 ng/spot. The method was validated for precision (inter and intraday), repeatability, and accuracy. Mean recovery was 100.30%. The relative standard deviation (RSD) values of the precision were found to be in the range 0.64—1.52%. In conclusion, the proposed TLC method was found to be precise, specific and accurate and can be used for identification and quantitative determination of L-dopa in herbal extract and its formulations.<ref>
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    Modi, K.P., Patel, N.M., Goyal, R.K. 2008. Estimation of L-Dopa from Mucuna pruriens LINN and Formulations Containing M. pruriens by HPTLC Method  ''Chemical and Pharmaceutical Bulletin''  56(3), 357-359. http://doi.org/10.1248/cpb.56.357</ref></blockquote>
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=Sources=
 
=Sources=
 
<references />
 
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Revision as of 16:49, 12 April 2016

AHPA recognizes other valuable resources exist regarding the identity of Mucuna pruriens.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Mucuna pruriens (L.) DC.   Fabaceae  
Standardized common name (English): velvet bean  
Ayurvedic name(s): atmagupta; kapikacchu

Botanical Voucher Specimen

bottomright bottomright bottomright

Mucuna pruriens (L.) DC. - Starr - 1686312-01.jpg
Mucuna pruriens (L.) DC.
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden[1]

Mucuna pruriens (L.) DC. - Starr - v-152-00989135.jpg
Mucuna pruriens (L.) DC.
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden[2]

Mucuna pruriens (L.) DC. - Starr - v-152-00989137.jpg
Mucuna pruriens (L.) DC.
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden[3]

Organoleptic Characteristics

Macroscopic Characteristics

[Mucuna pruriens] is a leguminous climbing plant ... The fruit is a coriaceous pod, shaped like the Italic letter f, about four inches long, and covered with brown bristly hairs, which easily separate, and when handled stick in the fingers, producing an intense itching sensation.

Source: United States Dispensatory (1918) [4]

Microscopic Characteristics

bottomright bottomright

Mucuna pruriens Alkemist Laboratories.jpg
Starch granules seen under polarized light showing observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[5]

Mucuna pruriens-1 Alkemist Laboratories.jpg
Small group of pigmented osteosclerieds observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[6]


High Performance Thin Layer Chromatographic Identification

Supplementary Information

Other Publications

HPTLC L-Dopa Estimation, Modi, et al., 2008

Estimation of L-Dopa from Mucuna pruriens LINN and Formulations Containing M. pruriens by HPTLC Method,

Abstract. A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the analysis of L-dopa in Mucuna pruriens seed extract and its formulations. The method involves densitometric evaluation of L-dopa after resolving it by HPTLC on silica gel plates with n-butanol–acetic acid–water (4.0+1.0+1.0, v/v) as the mobile phase. Densitometric analysis of L-dopa was carried out in the absorbance mode at 280 nm. The relationship between the concentration of L-dopa and corresponding peak areas was found to be linear in the range of 100 to 1200 ng/spot. The method was validated for precision (inter and intraday), repeatability, and accuracy. Mean recovery was 100.30%. The relative standard deviation (RSD) values of the precision were found to be in the range 0.64—1.52%. In conclusion, the proposed TLC method was found to be precise, specific and accurate and can be used for identification and quantitative determination of L-dopa in herbal extract and its formulations.[7]

Sources

  1. ↑ Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden http://sciweb.nybg.org/science2/VirtualHerbarium.asp
  2. ↑ Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden http://sciweb.nybg.org/science2/VirtualHerbarium.asp
  3. ↑ Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden http://sciweb.nybg.org/science2/VirtualHerbarium.asp
  4. ↑ United States Dispensatory (1918)
  5. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  6. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. ↑ Modi, K.P., Patel, N.M., Goyal, R.K. 2008. Estimation of L-Dopa from Mucuna pruriens LINN and Formulations Containing M. pruriens by HPTLC Method Chemical and Pharmaceutical Bulletin 56(3), 357-359. http://doi.org/10.1248/cpb.56.357
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