Phyllanthus emblica (fruit)

Microscopic Entries

Extremely large rosette/shards of calcium oxalate (some as small as a few 1μm in the form of sand) observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

HPTLC Entries

Phyllanthus emblica HPTLC ID - 10% Methanolic Sulfuric Acid UV 365 nm Amla (fruit) (Phyllanthus emblica) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 2 μL Gallic Acid ~0.1% in CH3OH 2 μL Phyllanthus emblica-1 2 μL Phyllanthus emblica-2 1 μL Phyllanthus emblica-3 1 μL Phyllanthus emblica-3 3 μL Phyllanthus emblica-4 3 μL Phyllanthus emblica-4 2 μL Gallic Acid ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase toluene: ethyl formate: formic acid [5/4/1]  Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method 10% Methanolic H2SO4 -> 115° C 15 min -> UV 365 nm  Reference see Indian Herbal Pharmacopoeia Vol 2 1999 Source: Elan M. Sudberg, Alkemist Laboratories