Piper methysticum (rhizome)

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(Created page with '=Introduction= =Macroscopic Entries= =Microscopic Entries= =HPTLC Entries= =Other Points of Interest= Category:blank')
 
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=Microscopic Entries=
 
=Microscopic Entries=
 
=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Kava (rhizome) (''Piper methysticum'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Piper_methysticum_-_Alkemists_Laboratories.jpg
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            | caption1=''Piper methysticum'' HPTLC ID - Vanillin Sulfuric Acid UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=H2O: acetonitrile: CH3OH: AcCOOH: >Reverse Phase!!!! [4/3/3/0.01/]
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            | prep=0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection=Vanillin/H2SO4  Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL Kavain
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# 3 μL ''Piper methysticum''-1 (rhizome)
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# 3 μL ''Piper methysticum''-2 (rhizome)
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# 3 μL ''Piper methysticum''-3 (rhizome)
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# 3 μL ''Piper methysticum''-3 (rhizome)
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# 3 μL ''Piper methysticum''-4 (rhizome)
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# 3 μL ''Piper methysticum''-5 (rhizome)
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# 3 μL Kavain
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 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Plant Drug Analysis, Wagner, H., 1996
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
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[[Category:blank]]

Revision as of 18:55, 23 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Piper methysticum HPTLC ID - Vanillin Sulfuric Acid UV 365 nm

Kava (rhizome) (Piper methysticum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Kavain
  2. 3 μL Piper methysticum-1 (rhizome)
  3. 3 μL Piper methysticum-2 (rhizome)
  4. 3 μL Piper methysticum-3 (rhizome)
  5. 3 μL Piper methysticum-3 (rhizome)
  6. 3 μL Piper methysticum-4 (rhizome)
  7. 3 μL Piper methysticum-5 (rhizome)
  8. 3 μL Kavain

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase H2O: acetonitrile: CH3OH: AcCOOH: >Reverse Phase!!!! [4/3/3/0.01/] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [1]

Other Points of Interest


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