Piper methysticum (rhizome)

HPTLC Entries

Piper methysticum HPTLC ID - Vanillin Sulfuric Acid UV 365 nm Kava (rhizome) (Piper methysticum) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Kavain 3 μL Piper methysticum-1 (rhizome) 3 μL Piper methysticum-2 (rhizome) 3 μL Piper methysticum-3 (rhizome) 3 μL Piper methysticum-3 (rhizome) 3 μL Piper methysticum-4 (rhizome) 3 μL Piper methysticum-5 (rhizome) 3 μL Kavain Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase H2O: acetonitrile: CH3OH: AcCOOH: >Reverse Phase!!!! [4/3/3/0.01/]  Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Plant Drug Analysis, Wagner, H., 1996 Source: Elan M. Sudberg, Alkemist Laboratories