Piper nigrum (fruit)

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=Botanical Voucher Specimen=
 
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            | source=MOBOT, Tropicos.org
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=Organoleptic Characteristics=
 
=Organoleptic Characteristics=

Revision as of 06:44, 26 March 2014

Contents

Nomenclature

Piper nigrum L.   Piperaceae  
Standardized common name (English): black pepper (with cortex); green peppercorn (green with cortex); white pepper (decorticated)  
Ayurvedic name(s): maricha

Botanical Voucher Specimen

bottomright

Piper nigrum Tropicos 100213130 (S).jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

Cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [2]
AP-LOGO-Laboratories Crop - Copy.jpg


bottomright bottomright

Organic Black Pepper Alkemist Laboratories.jpg
Yellow oil gland seen within the outer region of the mesocarp observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]

Organic Black Pepper-1 Alkemist Laboratories.jpg
Group of isodiametric stone cells observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[4]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Piper nigrum HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Black Pepper (fruit) (Piper nigrum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Piperine ~0.1% in CH3OH
  2. 3 μL Piper nigrum-1 (fruit)
  3. 3 μL Piper nigrum-2 (fruit)
  4. 3 μL Piper nigrum-3 (fruit)
  5. 3 μL Piper nigrum-3 (fruit)
  6. 3 μL Piper nigrum-4 (fruit)
  7. 3 μL Piper nigrum-5 (fruit)
  8. 3 μL Piperine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate [7/3] 

Sample Preparation Method 0.3g + 3mL 100% gr EtOH, sonicate/heat @ 50°C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005


Source: Elan M. Sudberg, Alkemist Laboratories [5]

Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100213130
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  5. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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