Piper nigrum (fruit)

From AHPA Botanical Identity References Compendium
(Difference between revisions)
Jump to: navigation, search
(add askbox)
(add Culbreth ref for organoleptic, macro, micro)
 
Line 25: Line 25:
  
 
=Organoleptic Characteristics=
 
=Organoleptic Characteristics=
 
+
{| border=1
 +
|
 +
{{Organolepsy | source=Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
 +
            | description=Odor aromatic, slightly empyreumatic; taste aromatic, very pungent.}}
 +
|}
 
=Macroscopic Characteristics=
 
=Macroscopic Characteristics=
 +
{| border=1
 +
|
 +
{{Macroscopy | source=Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
 +
| description='''Plant:''' Perennial evergreen, dioecious climber; stem 6-9 M. (20-30°) long, articulated, smooth, woody, joints thickened, from which adventitious rootlets arise for adhering to support or taking root in the soil; leaves 10-15 Cm. (4-6') long, ovate, petioles 12 Mm. (1/2') long, acute at base and apex, entire, smooth, leathery, dark green, 5-7-nerved; flowers whitish, unisexual, sessile, elongated spikes.
 +
 +
'''Fruit:''' berry-like, sessile, green, then red, and when ripe yellow, nearly globular, 3.5-6 Mm. (1/7 - 1/4') broad, epicarp very thin, easily separable from the sarcocarp, grayish-black, coarsely reticulate, unilocular, 1-seeded; seed nearly white, hollow, adhering to pericarp.
 +
}}
 +
|}
  
 
=Microscopic Characteristics=
 
=Microscopic Characteristics=
 
{| border=1
 
{| border=1
 
|
 
|
 +
{{Microscopy | source=Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
 +
| description=Powder grayish black, microscopically -- blackish-brown fragments of pericarp and nearly white of endosperm and embryo, starch grains 0.001-0.003 Mm. (1/25000 - 1/8300') broad, stone cells of epicarp and endocarp with reddish-brown substance, oil cells with yellowish oil from which piperine prisms may separate.
 +
}}
  
 
{{Microscopy | source= Elan M. Sudberg, Alkemist Laboratories
 
{{Microscopy | source= Elan M. Sudberg, Alkemist Laboratories

Latest revision as of 16:15, 7 July 2015

AHPA recognizes other valuable resources exist regarding the identity of Piper nigrum.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Piper nigrum L.   Piperaceae  
Standardized common name (English): black pepper (with cortex); green peppercorn (green with cortex); white pepper (decorticated)  
Ayurvedic name(s): maricha

Botanical Voucher Specimen

bottomright

Piper nigrum Tropicos 100213130 (S).jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Odor aromatic, slightly empyreumatic; taste aromatic, very pungent.

Source: Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed. [2]

Macroscopic Characteristics

Plant: Perennial evergreen, dioecious climber; stem 6-9 M. (20-30°) long, articulated, smooth, woody, joints thickened, from which adventitious rootlets arise for adhering to support or taking root in the soil; leaves 10-15 Cm. (4-6') long, ovate, petioles 12 Mm. (1/2') long, acute at base and apex, entire, smooth, leathery, dark green, 5-7-nerved; flowers whitish, unisexual, sessile, elongated spikes.

Fruit: berry-like, sessile, green, then red, and when ripe yellow, nearly globular, 3.5-6 Mm. (1/7 - 1/4') broad, epicarp very thin, easily separable from the sarcocarp, grayish-black, coarsely reticulate, unilocular, 1-seeded; seed nearly white, hollow, adhering to pericarp.

Source: Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed. [3]

Microscopic Characteristics

Powder grayish black, microscopically -- blackish-brown fragments of pericarp and nearly white of endosperm and embryo, starch grains 0.001-0.003 Mm. (1/25000 - 1/8300') broad, stone cells of epicarp and endocarp with reddish-brown substance, oil cells with yellowish oil from which piperine prisms may separate.

Source: Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed. [4]


Cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [5]

AP-LOGO-Laboratories Crop - Copy.jpg


bottomright bottomright

Organic Black Pepper Alkemist Laboratories.jpg
Yellow oil gland seen within the outer region of the mesocarp observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[6]

Organic Black Pepper-1 Alkemist Laboratories.jpg
Group of isodiametric stone cells observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[7]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Piper nigrum HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Black Pepper (fruit) (Piper nigrum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Piperine ~0.1% in CH3OH
  2. 3 μL Piper nigrum-1 (fruit)
  3. 3 μL Piper nigrum-2 (fruit)
  4. 3 μL Piper nigrum-3 (fruit)
  5. 3 μL Piper nigrum-3 (fruit)
  6. 3 μL Piper nigrum-4 (fruit)
  7. 3 μL Piper nigrum-5 (fruit)
  8. 3 μL Piperine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate [7/3] 

Sample Preparation Method 0.3g + 3mL 100% gr EtOH, sonicate/heat @ 50°C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005


Source: Elan M. Sudberg, Alkemist Laboratories [8]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100213130
  2. Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
  3. Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
  4. Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
  5. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  6. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  8. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
Personal tools
MediaWiki Appliance - Powered by TurnKey Linux