Piper nigrum (fruit)

Microscopic Entries

Yellow oil gland seen within the outer region of the mesocarp observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

HPTLC Entries

Piper nigrum HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm Black Pepper (fruit) (Piper nigrum) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Piperine ~0.1% in CH3OH 3 μL Piper nigrum-1 (fruit) 3 μL Piper nigrum-2 (fruit) 3 μL Piper nigrum-3 (fruit) 3 μL Piper nigrum-3 (fruit) 3 μL Piper nigrum-4 (fruit) 3 μL Piper nigrum-5 (fruit) 3 μL Piperine ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase toluene: ethyl acetate [7/3]  Sample Preparation Method 0.3g + 3mL 100% gr EtOH, sonicate/heat @ 50°C ~ 1/2 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005 Source: Elan M. Sudberg, Alkemist Laboratories