Rehmannia glutinosa (root)

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AHPA recognizes other valuable resources exist regarding the identity of Rehmannia glutinosa.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Rehmannia glutinosa Libosch. ex Fisch. & Mey.   Scrophulariaceae  
Standardized common name (English): rehmannia  
Pinyin name(s): di huang; sheng di huang (unprocessed root tuber); shu di huang (cured root tuber)

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

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Rehmannia glutinosa - Alkemist Laboratories.jpg
Parenchyma showing red pigmented material observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[1]

Rehmannia glutinosa-1 - Alkemist Laboratories.jpg
Vessels showing circular perforation observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

High Performance Thin Layer Chromatographic Identification

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Rehmannia glutinosa HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Rehmannia (root) (Rehmannia glutinosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Rehmannia glutinosa-1 (root)
  2. 3 μL Rehmannia glutinosa-2 (root)
  3. 3 μL Rehmannia glutinosa-3 (root)
  4. 3 μL Rehmannia glutinosa-4 (root)
  5. 3 μL Rehmannia glutinosa-4 (root)
  6. 3 μL Rehmannia glutinosa-5 (root)
  7. 3 μL Rehmannia glutinosa-6 (root)
  8. 2 μL Catalpol ~ 0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [7/3/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Japanese Official Monographs part III


Source: Elan M. Sudberg, Alkemist Laboratories [3]

Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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