Rosa canina (fruit)

From AHPA Botanical Identity References Compendium
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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Rosehips (fruit) (''Rosa canina'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Rosa_canina_-_Alkemists_Laboratories.jpg
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            | caption1=''Rosa canina'' HPTLC ID - Vanillin/Sulfuric Acid Visible Light
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=toluene: acetone: HCOOH [6/3/1]
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            | prep=0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 5 μL Ascorbic Acid ~0.1% in CH3OH
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# 3 μL ''Rosa canina''-1 (fruit)
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# 5 μL ''Rosa canina''-1 (fruit)
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# 4 μL ''Rosa canina''-2 (fruit)
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# 4 μL ''Rosa canina''-2 (fruit)
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# 4 μL ''Rosa canina''-3 (fruit)
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# 4 μL ''Rosa canina''-4 (fruit)
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# 5 μL Ascorbic Acid ~0.1% in CH3OH
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Adapted from European Pharmacopoeia 5.0 2005
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 20:33, 24 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Fragment of a unicellular thick walled trichome seen under polarized light observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Rosa canina - Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Rosa canina HPTLC ID - Vanillin/Sulfuric Acid Visible Light

Rosehips (fruit) (Rosa canina)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 5 μL Ascorbic Acid ~0.1% in CH3OH
  2. 3 μL Rosa canina-1 (fruit)
  3. 5 μL Rosa canina-1 (fruit)
  4. 4 μL Rosa canina-2 (fruit)
  5. 4 μL Rosa canina-2 (fruit)
  6. 4 μL Rosa canina-3 (fruit)
  7. 4 μL Rosa canina-4 (fruit)
  8. 5 μL Ascorbic Acid ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: acetone: HCOOH [6/3/1] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light 

Reference see Adapted from European Pharmacopoeia 5.0 2005


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Other Points of Interest


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