Rosmarinus officinalis (leaf)

Microscopic Entries

Branched covering trichomes from the lower surface of the leaf observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are branched covering trichome from the lower surface of the leaf and the large glandular scales) when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

HPTLC Entries

Rosmarinus officinalis HPTLC ID - Natural Product Reagent + PEG UV 365 nm Rosemary (leaf) (Rosmarinus officinalis) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Rosmarinic Acid ~0.1% in CH3OH 3 μL Rosmarinus officinalis-1 (leaf) 3 μL Rosmarinus officinalis-2 (leaf) 3 μL Rosmarinus officinalis-3 (leaf) 3 μL Rosmarinus officinalis-3 (leaf) 3 μL Rosmarinus officinalis-4 (leaf) 3 μL Rosmarinus officinalis-5 Vouchered Specimen (herb) 1 μL Caffeic acid, Chlorogenic Acid, Hyperoside, Rutin ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase toluene: ethyl formate: HCOOH [5/4/1]  Sample Preparation Method 0.3g+3mL 80% CH3OH sonicate/heat @ 50°C ~ 1/2 hr  Detection Method Natural Product Reagent + PEG -> UV 365 nm  Reference see British Pharmacopoeia, 2003 Source: Elan M. Sudberg, Alkemist Laboratories