Smilax aristolochiifolia (root)

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=Macroscopic Characteristics=
 
=Macroscopic Characteristics=
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{{Macroscopy | source=United States Dispensatory (1918)
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| description=''S. medica'' has an angular stem, armed with straight prickles at the joints, and a few hooked ones in the intervals. The leaves are smooth, bright green on both sides, shortly acuminate, five-nerved, with the veins prominent beneath. They vary much in form, the lower being cordate auriculate-hastate, the upper cordate-ovate. In the old leaves the petiole and midrib are armed with straight subulate prickles. The inflorescence is an umbel of from eight to twelve flowers, with a smooth axillary peduncle and pedicels about three lines long.
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The Mexican or Vera Cruz sarsaparilla is derived from this species. ... [The roots] consist of a head or caudex with numerous long radicles, which, however, are somewhat smaller than in that variety, and have a thinner bark. They are often also much coiled with earth. It contains but little starch and has quadrangular endodermal cells, with thickened walls, and more or less oval lumen.}}
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=Microscopic Characteristics=
 
=Microscopic Characteristics=
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{{Macroscopy | source=United States Dispensatory (1918)
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| description=Under the microscope, transverse sections of all of the commercial varieties of Sarsaparilla show an epidermal layer with basal portions of root hairs; a hypodermis composed of several layers of strongly lignified cells, the walls being uniformly thickened, except in Mexican Sarsaparilla in which the inner walls are only slightly thickened; a cortex composed of numerous parenchyma cells mostly containing starch, some containing resin or raphides of calcium oxalate; an endodermis of a single layer of strongly lignified cells, the walls being' uniformly thickened except in Mexican Sarsaparilla in which the outer walls are only slightly thickened; a central cylinder composed of radial bundles connected with sclerenchymatous fibers, the tracheas being large and oval and the phloem in small groups at the periphery of the bundle; and a pith composed of starch-bearing parenchyma cells. Powdered Sarsaparilla is light grayish-brown to dark grayish-brown; when examined under the microscope it exhibits numerous starch grains, from 0.003 to 0.023 mm. in diameter, spherical, or biconvex or spherical-tetrahedral, single to 2- to 4-compound, and frequently with a central-elliptical cleft; calcium oxalate in raphides, from 0.006 to 0.035 mm. in diameter, occurring singly or in groups; cells of the hypodermis and endodermis with lemon-yellow or reddish-yellow porous walls and, in the case of Mexican Sarsaparilla, showing an uneven or irregular thickening, the individual cells from 0.08 to 0.5 mm. in length; fragments of trachea) with simple and bordered pores or scalariform or reticulate thickenings associated with sclerenchymatous fibers having rather thin, very slightly lignified and porous walls. }}
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
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=High Performance Thin Layer Chromatographic Identification=
 
=High Performance Thin Layer Chromatographic Identification=
  

Revision as of 20:57, 7 April 2015

AHPA recognizes other valuable resources exist regarding the identity of Smilax aristolochiifolia.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Smilax aristolochiifolia Mill.   Smilacaceae  
Syn. Smilax medica Schltdl. & Cham.; Smilax ornata Lem.  
Standardized common name (English): sarsaparilla

Botanical Voucher Specimen

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Smilax aristolochiifolia Tropicos 26042.jpg
Source: MOBOT, Tropicos.org[1]

Smilax aristolochiifolia Tropicos 27626.jpg
Source: MOBOT, Tropicos.org[2]

Organoleptic Characteristics

Macroscopic Characteristics

S. medica has an angular stem, armed with straight prickles at the joints, and a few hooked ones in the intervals. The leaves are smooth, bright green on both sides, shortly acuminate, five-nerved, with the veins prominent beneath. They vary much in form, the lower being cordate auriculate-hastate, the upper cordate-ovate. In the old leaves the petiole and midrib are armed with straight subulate prickles. The inflorescence is an umbel of from eight to twelve flowers, with a smooth axillary peduncle and pedicels about three lines long.

The Mexican or Vera Cruz sarsaparilla is derived from this species. ... [The roots] consist of a head or caudex with numerous long radicles, which, however, are somewhat smaller than in that variety, and have a thinner bark. They are often also much coiled with earth. It contains but little starch and has quadrangular endodermal cells, with thickened walls, and more or less oval lumen.

Source: United States Dispensatory (1918) [3]

Microscopic Characteristics

Under the microscope, transverse sections of all of the commercial varieties of Sarsaparilla show an epidermal layer with basal portions of root hairs; a hypodermis composed of several layers of strongly lignified cells, the walls being uniformly thickened, except in Mexican Sarsaparilla in which the inner walls are only slightly thickened; a cortex composed of numerous parenchyma cells mostly containing starch, some containing resin or raphides of calcium oxalate; an endodermis of a single layer of strongly lignified cells, the walls being' uniformly thickened except in Mexican Sarsaparilla in which the outer walls are only slightly thickened; a central cylinder composed of radial bundles connected with sclerenchymatous fibers, the tracheas being large and oval and the phloem in small groups at the periphery of the bundle; and a pith composed of starch-bearing parenchyma cells. Powdered Sarsaparilla is light grayish-brown to dark grayish-brown; when examined under the microscope it exhibits numerous starch grains, from 0.003 to 0.023 mm. in diameter, spherical, or biconvex or spherical-tetrahedral, single to 2- to 4-compound, and frequently with a central-elliptical cleft; calcium oxalate in raphides, from 0.006 to 0.035 mm. in diameter, occurring singly or in groups; cells of the hypodermis and endodermis with lemon-yellow or reddish-yellow porous walls and, in the case of Mexican Sarsaparilla, showing an uneven or irregular thickening, the individual cells from 0.08 to 0.5 mm. in length; fragments of trachea) with simple and bordered pores or scalariform or reticulate thickenings associated with sclerenchymatous fibers having rather thin, very slightly lignified and porous walls.

Source: United States Dispensatory (1918) [4]

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Smilax aristolochiifolia - Alkemist Laboratories.jpg
Prismatic crystals of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[5]

Smilax aristolochiifolia-1 - Alkemist Laboratories.jpg
Dark orange resinous material from cork observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[6]

High Performance Thin Layer Chromatographic Identification

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/26042
  2. MOBOT, Tropicos.org http://www.tropicos.org/Image/27626
  3. United States Dispensatory (1918)
  4. United States Dispensatory (1918)
  5. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  6. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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