Giant goldenrod herb (aerial parts) HPTLC ID - NP reagent and PEG 400 reagent, UV 366 nm

Giant goldenrod herb (aerial parts) (Solidago gigantea)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 µL Giant goldenrod herb 1
2. 5 µL Giant goldenrod herb 1
3. 7 µL Giant goldenrod herb 1
4. 1 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
5. 3 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
6. 5 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
7. 5 µL Rutin
8. 5 µL Quercitrin
9. 5 µL Chlorogenic acid
10. 5 µL Giant goldenrod herb 2
11. 5 µL Goldenrod herb (old sample)
12. 7 µL Goldenrod herb (old sample)
13. 5 µL European goldenrod herb 1
14. 7 µL European goldenrod herb 1 

Reference Sample(s) Reference: Individually dissolve 1 mg of rutin and 1 mg of chlorogenic acid each in 1 mL of methanol. Optional: dissolve 1 mg of quercitrin in 1 mL of methanol. 

Stationary Phase Stationary phase, i.e. Silica gel 60, F254 

Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate for 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent. 

Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.30; Chlorogenic acid: green fluorescent zone at Rf ~ 0.48.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows an orange zone at Rf ~ 0.30 corresponding to reference substance rutin and a green zone at Rf ~ 0.48 corresponding to chlorogenic acid (pink arrows). Above it there are several faint brown zones and an intense brown zone (Rf ~ 0.67) at the position of quercitrin (yellow arrow). A green zone is seen at Rf ~ 0.82.

Test for other species: European goldenrod herb does not show an intense brown zone at Rf ~ 0.67.

Source: HPTLC Association ^[1]