Stellaria media (leaf)

From AHPA Botanical Identity References Compendium
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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Chickweed (leaf) (''Stellaria media'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Stellaria_media_-_Alkemists_Laboratories.jpg
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            | caption1=''Stellaria media'' HPTLC ID - Natural Product Reagent + PEG -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=ethyl acetate: formic acid: acetic acid: water [10/1/1/2]
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            | prep=0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection=Natural Product Reagent + PEG -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 2 μL Caffeic acid, Chlorogenic Acid, Hyperoside, Rutin ~0.1% in CH3OH
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# 3 μL ''Stellaria media''-1
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# 3 μL ''Stellaria media''-2
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# 3 μL ''Stellaria media''-3
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# 3 μL ''Stellaria media''-3
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# 3 μL ''Stellaria media''-4
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# 3 μL ''Stellaria media''-5
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# 3 μL ''Stellaria media''-6
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 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.
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            | reference=Plant Drug Analysis, Wagner, H., 1996
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 21:11, 22 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Sinuous anticlinal pigmented cell walls observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are sinuous anticlinal pigmented cell walls and the large cluster of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Chickweed herb Alkemist Laboratories.jpg



HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Stellaria media HPTLC ID - Natural Product Reagent + PEG -> UV 365 nm

Chickweed (leaf) (Stellaria media)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Caffeic acid, Chlorogenic Acid, Hyperoside, Rutin ~0.1% in CH3OH
  2. 3 μL Stellaria media-1
  3. 3 μL Stellaria media-2
  4. 3 μL Stellaria media-3
  5. 3 μL Stellaria media-3
  6. 3 μL Stellaria media-4
  7. 3 μL Stellaria media-5
  8. 3 μL Stellaria media-6

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: formic acid: acetic acid: water [10/1/1/2] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Other Points of Interest


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