Stellaria media (leaf)

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Revision as of 02:42, 16 March 2014

Contents

Nomenclature

Stellaria media (L.) Vill.   Caryophyllaceae  
Standardized common name (English): chickweed

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

Sinuous anticlinal pigmented cell walls observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are sinuous anticlinal pigmented cell walls and the large cluster of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Chickweed herb Alkemist Laboratories.jpg



High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Stellaria media HPTLC ID - Natural Product Reagent + PEG -> UV 365 nm

Chickweed (leaf) (Stellaria media)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Caffeic acid, Chlorogenic Acid, Hyperoside, Rutin ~0.1% in CH3OH
  2. 3 μL Stellaria media-1
  3. 3 μL Stellaria media-2
  4. 3 μL Stellaria media-3
  5. 3 μL Stellaria media-3
  6. 3 μL Stellaria media-4
  7. 3 μL Stellaria media-5
  8. 3 μL Stellaria media-6

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: formic acid: acetic acid: water [10/1/1/2] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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