Stellaria media (leaf)

Nomenclature

Stellaria media (L.) Vill.   Caryophyllaceae  
Standardized common name (English): chickweed

Microscopic Characteristics

Sinuous anticlinal pigmented cell walls observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories	Large cluster of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Stellaria media HPTLC ID - Natural Product Reagent + PEG -> UV 365 nm Chickweed (leaf) (Stellaria media) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 2 μL Caffeic acid, Chlorogenic Acid, Hyperoside, Rutin ~0.1% in CH3OH 3 μL Stellaria media-1 3 μL Stellaria media-2 3 μL Stellaria media-3 3 μL Stellaria media-3 3 μL Stellaria media-4 3 μL Stellaria media-5 3 μL Stellaria media-6 Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase ethyl acetate: formic acid: acetic acid: water [10/1/1/2]  Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method Natural Product Reagent + PEG -> UV 365 nm  Reference see Plant Drug Analysis, Wagner, H., 1996 Source: Elan M. Sudberg, Alkemist Laboratories