Uncaria tomentosa (root)

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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Cat’s Claw (bark) (''Uncaria tomentosa'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Uncaria_tomentosa_bark_-_Alkemists_Laboratories.jpg
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            | caption1=’’Uncaria tomentosa’’ HPTLC ID - Natural Product Reagent + PEG-> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase= ethyl acetate: CH3OH: HCOOH: H2O [10/0.27/0.3/0.5]
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            | prep= 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection= Natural Product Reagent + PEG-> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL Uncarine C~0.1% in CH3OH
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# 3 μL ''Uncaria tomentosa''-1 (bark)
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# 3 μL ''Uncaria tomentosa''-2 (bark)
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# 3 μL ''Uncaria tomentosa''-3 (bark)
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# 3 μL ''Uncaria tomentosa''-3 (bark)
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# 3 μL ''Uncaria tomentosa''-4 (bark)
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# 3 μL ''Uncaria tomentosa''-5 (bark)
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# 3 μL Uncarine E~0.1% in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Plant Drug Analysis, Wagner, H., 1996
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:NoIntro]]
 
[[Category:NoIntro]]

Revision as of 21:51, 15 March 2013

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Group of parallel heavily lignified fibers observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Uncaria tomentosa - Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
’’Uncaria tomentosa’’ HPTLC ID - Natural Product Reagent + PEG-> UV 365 nm

Cat’s Claw (bark) (Uncaria tomentosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Uncarine C~0.1% in CH3OH
  2. 3 μL Uncaria tomentosa-1 (bark)
  3. 3 μL Uncaria tomentosa-2 (bark)
  4. 3 μL Uncaria tomentosa-3 (bark)
  5. 3 μL Uncaria tomentosa-3 (bark)
  6. 3 μL Uncaria tomentosa-4 (bark)
  7. 3 μL Uncaria tomentosa-5 (bark)
  8. 3 μL Uncarine E~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: CH3OH: HCOOH: H2O [10/0.27/0.3/0.5] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG-> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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