Uncaria tomentosa HPTLC ID - Natural Product Reagent + PEG-> UV 365 nm

Cat’s Claw (bark) (Uncaria tomentosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Uncarine C~0.1% in CH3OH
2. 3 μL Uncaria tomentosa-1 (bark)
3. 3 μL Uncaria tomentosa-2 (bark)
4. 3 μL Uncaria tomentosa-3 (bark)
5. 3 μL Uncaria tomentosa-3 (bark)
6. 3 μL Uncaria tomentosa-4 (bark)
7. 3 μL Uncaria tomentosa-5 (bark)
8. 3 μL Uncarine E~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: CH3OH: HCOOH: H2O [10/0.27/0.3/0.5] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG-> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]