Uncaria tomentosa (root)

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=Microscopic Characteristics=
 
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{{Media |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
{{Media |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories

Latest revision as of 17:09, 28 April 2015

AHPA recognizes other valuable resources exist regarding the identity of Uncaria tomentosa.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Uncaria tomentosa (Willd.) DC.   Rubiaceae  
Standardized common name (English): cat's claw

Botanical Voucher Specimen

bottomright bottomright

Uncaria tomentosa Tropicos 18534.jpg
Source: MOBOT, Tropicos.org[1]

Uncaria tomentosa Tropicos 18535.jpg
Source: MOBOT, Tropicos.org[2]

Organoleptic Characteristics

Macroscopic Characteristics

bottomright bottomright

PlantaPhile - 2412.jpg
Source: PlantaPhile[3]

PlantaPhile - 3052.jpg
Source: PlantaPhile[4]


Microscopic Characteristics

bottomright

Uncaria tomentosa - Alkemist Laboratories.jpg
Group of parallel heavily lignified fibers observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[5]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Uncaria tomentosa HPTLC ID - Natural Product Reagent + PEG-> UV 365 nm

Cat’s Claw (bark) (Uncaria tomentosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Uncarine C~0.1% in CH3OH
  2. 3 μL Uncaria tomentosa-1 (bark)
  3. 3 μL Uncaria tomentosa-2 (bark)
  4. 3 μL Uncaria tomentosa-3 (bark)
  5. 3 μL Uncaria tomentosa-3 (bark)
  6. 3 μL Uncaria tomentosa-4 (bark)
  7. 3 μL Uncaria tomentosa-5 (bark)
  8. 3 μL Uncarine E~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: CH3OH: HCOOH: H2O [10/0.27/0.3/0.5] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG-> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [6]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/18534
  2. MOBOT, Tropicos.org http://www.tropicos.org/Image/18535
  3. PlantaPhile http://plantaphile.com/
  4. PlantaPhile http://plantaphile.com/
  5. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  6. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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