Arnebia spp. (root)

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AHPA recognizes other valuable resources exist regarding the identity of Arnebia spp..

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Botanical Voucher Specimen

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Arnebia decumbens f. micrantha Kuntze - Starr - 00334952.jpg
Arnebia decumbens f. micrantha Kuntze
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden[1]

Arnebia decumbens var. erecta Kuntze - Starr - 00334951.jpg
Arnebia decumbens var. erecta Kuntze
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden[2]

Organoleptic Characteristics

Macroscopic Characteristics

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Arnebia guttata Tropicos 100165538.jpg
Arnebia guttata - Tropicos.org (Flora of Pakistan)
Source: MOBOT, Tropicos.org[3]

Arnebia euchroma Tropicos 100165539.jpg
Arnebia euchroma - Tropicos.org (Flora of Pakistan)
Source: MOBOT, Tropicos.org[4]

Microscopic Characteristics

High Performance Thin Layer Chromatographic Identification

HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
(thumbnail)
Arnebia root, zi cao (root) HPTLC ID - UV 366 nm

Arnebia root, zi cao (root) (Arnebia euchroma or Arnebia guttata)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 µL Arnebia root 1
  2. 5 µL Arnebia root 1
  3. 7 µL Arnebia root 1
  4. 7 µL Arnebia root 2
  5. 7 µL Arnebia root 3
  6. 3 µL CAMAG test dye mixture III
  7. 7 µL Arnebia root 4 (old sample)
  8. 7 µL Yunnan onosma root
  9. 7 µL Tibetan onosma root 

Reference Sample(s) Reference: Dilute CAMAG test dye mixture III 1:10 with toluene. 

Stationary Phase Stationary phase, i.e. Silica gel 60, RP-18 

Mobile Phase acetone, 5% aqueous formic acid 8:2 (v/v) 

Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. 

Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test (under white RT): CAMAG test dye mixture III: A blue zone: at Rf ~ 0.28 and an orange red double zone at Rf ~ 0.48.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 366 nm the chromatogram of the test solution shows a blue zone right above the application position, a diffuse red zone at Rf ~ 0.35, above it another blue, a red and a purple zone and a characteristic blue zone at Rf ~ 0.71. Under white RT there is an intense and diffuse purple zone at Rf ~ 0.35. Above it there is a purple zone at Rf ~ 0.50 and at Rf ~ 0.60. A blue zone is visible at Rf ~ 0.67.

Test for adulteration: Under white RT no zones are seen at Rf ~ 0.60 (black arrow) and Rf ~ 0.67 (red arrow) (Yunnan onosma root, Tibetan onosma root, or low quality/old Arnebia root).

Source: HPTLC Association [5]

Supplementary Information

Sources

  1. Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden http://sciweb.nybg.org/science2/VirtualHerbarium.asp
  2. Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden http://sciweb.nybg.org/science2/VirtualHerbarium.asp
  3. MOBOT, Tropicos.org http://www.tropicos.org/Image/100165538
  4. MOBOT, Tropicos.org http://www.tropicos.org/Image/100165539
  5. HPTLC Association http://www.hptlc-association.org/
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