Citrus paradisi (seed)

Nomenclature

Citrus x paradisi Macfad.   Rutaceae  
Standardized common name (English): grapefruit

Botanical Voucher Specimen

Citrus paradisi Macfad. Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden

High Performance Thin Layer Chromatographic Identification

Citrus paradisi HPTLC ID - 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm Grapefruit (seed) (Citrus paradisi) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Hesperidin, Hesperitin ~0.1% in CH3OH 3 μL Citrus paradisi-1 (seed) 3 μL Citrus paradisi-2 (seed) 3 μL Citrus paradisi-3 (seed) 3 μL Citrus paradisi-3 (seed) 3 μL Citrus paradisi-4 (seed) 3 μL Citrus paradisi-5 (seed) 3 μL Naringin, Naringenin ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase CHCl3: CH3OH: HCOOH: H2O [6/2/1.4/0.1]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm  Reference see Method Developed by Alkemists Laboratories Source: Elan M. Sudberg, Alkemist Laboratories

Supplementary Information

ABC-AHP-NCNPR Botanical Adulterants Prevention Program Laboratory Guidance

Grapefruit (seed extract) (Citrus paradisi) 

General Characteristics This ABC-AHP-NCNPR Botanical Adulterants Prevention Program Laboratory Guidance Document presents an overview and comparison of various analytical technologies and methods used to differentiate between authentic grapefruit seed extract ingredients and non-authentic materials, with particular discussion of synthetic microbicidal compounds identified in non-authentic materials, authentic grapefruit seed extract components, and a detailed literature review of methods (including sample characterization via TLC, HPLC-UV, HPLC-MS, GC-MS, and others).

Source: ABC-AHP-NCNPR Botanical Adulterants Prevention Program