Euphrasia stricta (aerial parts)

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Euphrasia stricta J.P. Wolff ex J.F. Lehm.   Scrophulariaceae  
Syn. Euphrasia officinalis  
Standardized common name (English): eyebright

Botanical Voucher Specimen

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Euphrasia officinalis Tropicos 100285389.jpg
Source: MOBOT,[1]

Euphrasia officinalis Kew imageBarcode=K000195686 98404.jpg
Source: Royal Botanic Gardens, Kew.[2]

Organoleptic Characteristics

E. officinalis is a small annual plant without odor, and of a bitterish, astringent taste.

Source: United States Dispensatory (1918) [3]

Macroscopic Characteristics

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PlantaPhile - 3012.jpg
Source: PlantaPhile[4]

PlantaPhile - 2644.jpg
Source: PlantaPhile[5]

Microscopic Characteristics

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
Euphrasia officinalis HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm

Eyebright (aerial parts) (Euphrasia officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Aucubin ~0.1% in CH3OH
  2. 3 μL Euphrasia officinalis-1 (aerial parts)
  3. 3 μL Euphrasia officinalis-2 (aerial parts)
  4. 3 μL Euphrasia officinalis-3 (aerial parts)
  5. 3 μL Euphrasia officinalis-3 (aerial parts)
  6. 3 μL Euphrasia officinalis-4 (aerial parts)
  7. 3 μL Euphrasia officinalis-5 (aerial parts)
  8. 2 μL Catalpol ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6/4/0.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004

Source: Elan M. Sudberg, Alkemist Laboratories [6]

Supplementary Information


  1. MOBOT,
  2. Royal Botanic Gardens, Kew.
  3. United States Dispensatory (1918)
  4. PlantaPhile
  5. PlantaPhile
  6. Elan M. Sudberg, Alkemist Laboratories
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