Medicago sativa (leaf)

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Medicago sativa L.   Fabaceae  
Standardized common name (English): alfalfa

Botanical Voucher Specimen

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Medicago sativa Tropicos 100136132 (S).jpg
Source: MOBOT,[1]

Medicago sativa Kew barcode=K000226899 16978.jpg
Source: Royal Botanic Gardens, Kew.[2]

Medicago sativa Kew barcode=K001122610 708349.jpg
Source: Royal Botanic Gardens, Kew.[3]

Organoleptic Characteristics

Macroscopic Characteristics

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PlantaPhile - 116.jpg
Source: PlantaPhile[4]

PlantaPhile - 3044.jpg
Source: PlantaPhile[5]

Microscopic Characteristics

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Alfalfa Alkemist Laboratories.jpg
Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[6]

Alfalfa-1 Alkemist Laboratories.jpg
Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[7]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
Medicago sativa L. HPTLC ID - Ninhydrin Reagent, Visible Light

Alfalfa (leaf) (Medicago sativa L.)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Arginine ~0.1% in CH3OH
  2. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  3. 3 μL ‘‘Medicago sativa’’ L. (herb)
  4. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  5. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  6. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  7. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  8. 3 μL Canavanine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Ninhydrin Reagent -> visible light 

Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198

Source: Elan M. Sudberg, Alkemist Laboratories [8]

Supplementary Information


  1. MOBOT,
  2. Royal Botanic Gardens, Kew.
  3. Royal Botanic Gardens, Kew.
  4. PlantaPhile
  5. PlantaPhile
  6. Elan M. Sudberg, Alkemist Laboratories
  7. Elan M. Sudberg, Alkemist Laboratories
  8. Elan M. Sudberg, Alkemist Laboratories
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