Plantago lanceolata (leaf)

Nomenclature

Plantago lanceolata L.   Plantaginaceae  
Standardized common name (English): English plantain

Botanical Voucher Specimen

Source: MOBOT, Tropicos.org	Source: Botanical Voucher Specimen Library, Alkemists Laboratories	Source: Botanical Voucher Specimen Library, Alkemists Laboratories

Microscopic Characteristics

Anomocytic stomate next to a diacytic stomate observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories	Sheathed trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Ribwort plantain (leaf) HPTLC ID - Chloramine - trichloroacetic acid, UV 366 nm Ribwort plantain (leaf) (Plantago lanceolata) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 4 µl Ribwort plantain leaf 1 8 µl Ribwort plantain leaf 1 16 µl Ribwort plantain leaf 1 4 µl Ribwort plantain leaf 1 (extracted by shaking) 8 µl Ribwort plantain leaf 1 (extracted by shaking) 16 µl Ribwort plantain leaf 1 (extracted by shaking) 5 µl Aucubin 10 µl Acteoside 4 µl Grecian foxglove leaf 1 8 µl Grecian foxglove leaf 1 16 µl Grecian foxglove leaf 1 5 µl Grecian foxglove leaf 2 5 µl Grecian foxglove leaf 3 5 µl Grecian foxglove leaf 4 10 µl Greater plantain leaf  Reference Sample(s) Reference: Dissolve 1 mg of aucubin in 10 mL of methanol. Dissolve 1 mg of acteoside (verbascoside) in 10 mL of methanol  Stationary Phase Stationary phase, i.e. Silica gel 60, F254  Mobile Phase Ethyl acetate, methanol, water 81:11:8 (v/v/v)  Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Derivatization reagent: Chloramine - trichloroacetic acid reagent; Preparation: Solution A: Dissolve 300 mg of sodium-N-chloro-(4-methylbenzene) sulfonamide in 10 mL of water. Solution B: Dissolve 10 g of trichloroacetic acid in ethanol and fill up to a volume of 40 mL. Mix solution A and B and shake. Use: Dip (time 0, speed 5), heat at 100°C for 8 min.  Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%  Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes. System suitability test: Aucubin: black zone at Rf ~ 0.10 (white RT) Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under white RT the chromatogram of the test solution shows a black zone at Rf ~ 0.10 corresponding to aucubin; under UV 366 nm this zone appears brown (red arrows). Under UV 366 nm there is a blue zone at Rf ~ 0.35 and a red zone due to chlorophylls right below the solvent front. Test for adulteration: Under UV 366 nm no brown zone is seen at Rf ~ 0.10; under white RT no black zone is seen at Rf ~ 0.10 (Grecian foxglove leaf, Greater plantain leaf). Ribwort plantain (leaf) HPTLC ID - Chloramine - trichloroacetic acid, UV 366 nm Ribwort plantain(leaf) HPTLC ID - Chloramine - trichloroacetic acid, white RT Source: HPTLC Association

Supplementary Information

Plantago (leaf) (Plantago lanceolata) 

General Characteristics AHPA recommends in its Known Adulterants list that appropriate steps be taken to assure that this raw material is free of the noted adulterant. Contact AHPA for additional information regarding relevant analytical methods or follow this link for more information. 

Reported Adulterants Digitalis lanata leaf.

Source: AHPA Known Adulterants