Plantago ovata (seed husk)

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AHPA recognizes other valuable resources exist regarding the identity of Plantago ovata.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Plantago ovata Forssk.   Plantaginaceae  
Syn. Plantago ispaghula Roxb. ex Fleming  
Standardized common name (English): Indian plantain

Botanical Voucher Specimen

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Plantago ovata Tropicos 100186592 (S).jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

No odor or taste.

Source: United States Dispensatory (1918) [2]

Macroscopic Characteristics

Plantago ovata ... is a herbaceous plant ... either erect or decumbent and varies in height from 2.5 cm. to 1 dm. The leaves are ovate and either glabrous or shaggy hairy. The flower spikes are either cylindrical or reduced to a globular head.

Seeds boat-shaped, somewhat acute at one end, from two to three millimetres long and from one to one and a half millimetres wide; pale greyish-brown, with a darker elongated spot on the convex side; on the concave side the hilum covered with the remains of a thin white membrane. In water the testa swells, producing a viscous mucilage.

Source: United States Dispensatory (1918) [3]

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PlantaPhile - 2605.jpg
Source: PlantaPhile[4]

Microscopic Characteristics

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Psyllium Husk.jpg
Epidermal cells of the husks in surface view containing mucilage observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[5]

Psyllium Husk-1.jpg
Parenchyma cells seen under polarized light observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[6]


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Plantago ovata HPTLC ID - Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light

Psyllium (husk) (Plantago ovata)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Arabinose~0.1% in CH3OH
  2. 3 μL Plantago ovata-1 (husk)
  3. 5 μL Plantago ovata-1 (husk)
  4. 4 μL Plantago ovata-2 (husk)
  5. 4 μL Plantago ovata-2 (husk)
  6. 5 μL Plantago ovata-3 (husk)
  7. 3 μL Plantago ovata-3 (husk)
  8. 3 μL Galactose~0.1% in CH3OH


Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: methanol: water [6/1.5/1.5/1] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [7]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100186592
  2. United States Dispensatory (1918)
  3. United States Dispensatory (1918)
  4. PlantaPhile http://plantaphile.com/
  5. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  6. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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