Solidago virgaurea (aerial parts)

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Solidago virgaurea L.   Asteraceae  
Standardized common name (English): goldenrod

Botanical Voucher Specimen

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Solidago virgaurea Tropicos 100269718 (S).jpg
Source: MOBOT,[1]

Solidago virgaurea Tropicos 100269736 (S).jpg
Source: MOBOT,[2]

Organoleptic Characteristics

Macroscopic Characteristics

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PlantaPhile - 891.jpg
Source: PlantaPhile[3]

PlantaPhile - 2553.jpg
Source: PlantaPhile[4]

Microscopic Characteristics

High Performance Thin Layer Chromatographic Identification

European goldenrod herb (aerial parts) HPTLC ID - NP reagent and PEG reagent, UV 366 nm

European goldenrod herb (aerial parts) (Solidago virgaurea)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 µL Giant goldenrod herb 1
  2. 5 µL Giant goldenrod herb 1
  3. 7 µL Giant goldenrod herb 1
  4. 1 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
  5. 3 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
  6. 5 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
  7. 5 µL Rutin
  8. 5 µL Quercitrin
  9. 5 µL Chlorogenic acid
  10. 5 µL Giant goldenrod herb 2
  11. 5 µL Goldenrod herb (old sample)
  12. 7 µL Goldenrod herb (old sample)
  13. 5 µL European goldenrod herb 1
  14. 7 µL European goldenrod herb 1 

Reference Sample(s) Reference: Individually dissolve 1 mg of rutin and 1 mg of chlorogenic acid each in 1 mL of methanol. Optional: dissolve 1 mg of quercitrin in 1 mL of methanol. 

Stationary Phase Stationary phase, i.e. Silica gel 60, F254 

Mobile Phase Formic acid, water, methyl ethyl ketone, ethyl acetate 6:6:18:30 (v/v/v/v) 

Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate for 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent. 

Detection Method Saturated chamber; Developing distance 70 mm from lower edge; Relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.30; Chlorogenic acid: green fluorescent zone at Rf ~ 0.48.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows an orange zone at Rf ~ 0.30 corresponding to reference substance rutin and a green zone at Rf ~ 0.48 corresponding to chlorogenic acid (pink arrows). Above it there are two faint brown zones, the upper one at Rf ~ 0.67 at the position of reference quercitrin (faint brown zone). Another green zone is seen at Rf ~ 0.82.

Test for other species: No intense orange zone is seen at Rf ~ 0.67 (Giant goldenrod herb).

Source: HPTLC Association [5]

Supplementary Information


  1. MOBOT,
  2. MOBOT,
  3. PlantaPhile
  4. PlantaPhile
  5. HPTLC Association
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