Triticum aestivum (leaf)

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AHPA recognizes other valuable resources exist regarding the identity of Triticum aestivum.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Triticum aestivum L.   Poeceae  
Standardized common name (English): wheat

Botanical Voucher Specimen

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Triticum aestivum Tropicos 100173767.jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

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Triticum aestivum - Alkemist Laboratories.jpg
Unicellular thick walled tapering trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Triticum aestivum-1 - Alkemist Laboratories.jpg
Epidermis showing long thin stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Triticum aestivum HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Wheat grass (grass) (Triticum aestivum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Aescin~0.1% in CH3OH
  2. 2 μL Triticum aestivum-1 (grass)
  3. 4 μL Triticum aestivum-1 (grass)
  4. 3 μL Triticum aestivum-2 (grass)
  5. 3 μL Triticum aestivum-2 (grass)
  6. 3 μL Triticum aestivum-3 (grass)
  7. 3 μL Triticum aestivum-4 (grass)
  8. 3 μL Aescin~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6.4/5/1] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [4]


Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100173767
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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